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Controlling the separation of native proteins with temperature in thermal gel transient isotachophoresis.
Anal Bioanal Chem
July 2023
Department of Chemistry, Wayne State University, 5101 Cass Ave, Detroit, MI, 48202, USA.
Polyacrylamide gel electrophoresis (PAGE) is a ubiquitous technique used in biochemical research laboratories to characterize protein samples. Despite its popularity, PAGE is relatively slow and provides limited separation resolution, especially for native proteins. This report describes the development of a microfluidic thermal gel transient isotachophoresis (TG-tITP) method to rapidly separate native proteins with high resolution.
View Article and Find Full Text PDFA versatile and low-cost chip-to-world interface: Enabling ICP-MS characterization of isotachophoretically separated lanthanides on a microfluidic device.
Anal Chim Acta
November 2020
Pacific Northwest National Laboratory, P.O. Box 999, Richland, WA, 99352, USA. Electronic address:
Microfluidics offer novel and state-of-the-art pathways to process materials. Microfluidic systems drastically reduce timeframes and costs associated with traditional lab-scale efforts in the area of analytical sample preparations. The challenge arises in effectively connecting microfluidics to off-chip analysis tools to accurately characterize samples after treatment on-chip.
View Article and Find Full Text PDFCharacterizing the impact of thermal gels on isotachophoresis in microfluidic devices.
Electrophoresis
May 2020
Department of Chemistry, Wayne State University.
Thermally reversible Pluronic gels have been employed as separation matrices in microfluidic devices in the analysis of biological macromolecules. The phase of these gels can be tuned between liquid and solid states using temperature to vary fluidic resistance and alter peak resolution. Although separations in thermal gels have been characterized, their effect on isotachophoresis has not.
View Article and Find Full Text PDFSpatially Resolved Genetic Analysis of Tissue Sections Enabled by Microscale Flow Confinement Retrieval and Isotachophoretic Purification.
Angew Chem Int Ed Engl
October 2019
IBM Research-Zurich, Säumerstrasse 4, 8803, Rüschlikon, Switzerland.
We have developed a method for spatially resolved genetic analysis of formalin-fixed paraffin-embedded (FFPE) cell block and tissue sections. This method involves local sampling using hydrodynamic flow confinement of a lysis buffer, followed by electrokinetic purification of nucleic acids from the sampled lysate. We characterized the method by locally sampling an array of points with a circa 200 μm diameter footprint, enabling the detection of single KRAS and BRAF point mutations in small populations of RKO and MCF-7 FFPE cell blocks.
View Article and Find Full Text PDFMeasurement of recombinant human arylsulfatase A and leukocyte sulfatase activities by analytical isotachophoresis.
J Chromatogr B Analyt Technol Biomed Life Sci
August 2019
Department of Pediatric Neurology, University Children's Hospital Tübingen, Tübingen, Germany. Electronic address:
Metachromatic Leukodystrophy (MLD) and Multiple Sulfatase Deficiency (MSD) are rare and ultra-rare lysosomal storage diseases. Due to enzyme defects, patients are unable to split the sulfategroup from the respective substrates. In MSD all sulfatases are affected due to a defect of the Sulfatase Modifying Factor 1 (SUMF1) gene coding for the formylglycine generating enzyme (FGE) necessary for the modification of the active site of sulfatases.
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