Retention data methods for the determination of drug-protein binding parameters by high-performance liquid chromatography.

The binding to human serum albumin of some drugs (warfarin, furosemide and phenylbutazone) has been studied by high-performance liquid chromatography. Two methods have been used and compared, based on the measurement of the ligand retention volume under different conditions. The obtained total affinities of the drugs for the protein are in accordance with our previous results. The equilibrium saturation method leads easily to ni and Ki parameters from the retention volume of the ligand.