Protoplasts of the entomopathogenic bacterium Bacillus thuringiensis subsp. galleria were transformed by plasmid pBC16. The frequency of transformation was much lower than that of Bacillus subtilis. All isolated B. thuringiensis transformants were characterized by increased sensitivity to lysozyme as compared with the original strain.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC217044PMC
http://dx.doi.org/10.1128/jb.146.1.7-9.1981DOI Listing

Publication Analysis

Top Keywords

bacillus thuringiensis
8
thuringiensis subsp
8
subsp galleria
8
plasmid pbc16
8
transformation bacillus
4
galleria protoplasts
4
protoplasts plasmid
4
pbc16 protoplasts
4
protoplasts entomopathogenic
4
entomopathogenic bacterium
4

Similar Publications

The Cry2Aa protein is not enough to pose a threat to Pardosa astrigera.

Int J Biol Macromol

January 2025

Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China; National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China. Electronic address:

The widespread commercialization of genetically modified (GM) crops makes it important to assess the potential impact of Bacillus thuringiensis (Bt) on non-target organisms. Pardosa astrigera is an important predator in agroforestry ecosystems, and female and male spiders may react differently to Bt toxins due to their different activity habits and nutritional requirements. In this study, we found that exposure to Cry2Aa protein did not affect the survival and body weight of P.

View Article and Find Full Text PDF

Bioactive Potential of Some Strains from Macapá, Amazon, Brazil, Against the Housefly (Diptera: Muscidae) Under Laboratory Conditions.

Insects

December 2024

Programa de Pós-Graduação em Biodiversidade e Saúde, Instituto Oswaldo Cruz-Fundação Oswaldo Cruz (IOC/FIOCRUZ), Rio de Janeiro 21040-900, RJ, Brazil.

The high pathogenic activity of () strains against various insect orders has positioned it as the most effective, environmentally safe, and sustainable approach to integrated insect management. We aimed to identify strains capable of effectively controlling the housefly , a species of significant medical, veterinary, and public health concern. Twelve strains from Macapá, Brazil, were tested against housefly larvae.

View Article and Find Full Text PDF

Cell-to-Cell Natural Transformation Mediated Efficient Plasmid Transfer Between Species.

Int J Mol Sci

January 2025

State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.

Horizontal gene transfer (HGT) plays a pivotal role in bacterial evolution, shaping the genetic diversity of bacterial populations. It can occur through mechanisms such as conjugation, transduction, and natural transformation. , a model Gram-positive bacterium, serves not only as a robust system for studying HGT but also as a versatile organism with established industrial applications, such as producing industrial enzymes, antibiotics, and essential metabolites.

View Article and Find Full Text PDF

NupR Is Involved in the Control of PlcR: A Pleiotropic Regulator of Extracellular Virulence Factors.

Microorganisms

January 2025

Department of Microbiology, College of Life Sciences, Nankai University, Tianjin 300071, China.

NupR is a nucleoside permease regulator belonging to the GntR family, mainly regulating nucleoside transport in . A conserved binding site for NupR was found in the promoter region of . This study aimed to investigate the regulation of the virulence regulator PlcR by NupR and its impact on Bt virulence.

View Article and Find Full Text PDF

Cry1A Insecticidal Toxins and Their Digests Do Not Stimulate Histamine Release from Cultured Rat Mast Cells.

Biology (Basel)

December 2024

Graduate School of Science and Technology, Niigata University, 8050 Ikarashi 2 No-Cho, Nishi-ku, Niigata 950-2181, Japan.

Public acceptance of genetically modified crops engineered with (Bt) insecticidal protein genes (BT-GMCs), which confer resistance to various lepidopteran insect pests, is generally lacking. As a major concern over BT-GMCs is the allergenicity of insecticidal proteins, alleviating safety concerns should help increase public acceptance. In this study, three lepidopteran-specific Bt toxins, Cry1Aa, Cy1Ab, and Cry1Ac, were treated with simulated digestive fluids under various conditions.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!