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The paper deals with the redox state of free nicotinamide adenine dinucleotides (the NAD+/NADH ratio) in main compartments of the rat and guinea pig liver cells. NAD-pairs of cytoplasm and mitochondria in guinea pigs liver are shown to be more reduced than those in rats' liver. Stimulation of glucogenesis decreases the NAD+/NADH ratio in both compartments of rat liver and increases it in the guinea pigs' liver mitochondria. The guinea pigs' liver mitochondria synthesize actively phosphoenol pyruvate from oxaloacetate, malate and alpha-ketoglutarate. A decrease in the NAD+/NADH ratio value with introduction of beta-oxybutyrate into the incubation medium inhibits the phosphenolpyruvate synthesis from malate by 73%.
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