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Biochemical properties of immobilized horseradish peroxidase on ceramic and its application in removal of azo dye.
Sci Rep
November 2024
Molecular Biology Department, National Research Centre, Dokki, Cairo, Egypt.
In the current work, electrostatic interactions were used to immobilize the horseradish peroxidase (HRP) onto five types of ceramic materials (C) with different concentrations of oxidized metals (C1-C5). The highest immobilization efficiency (70 and 77%) was detected at 6 mg C3 and 18 enzyme units. Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX) and Fourier Transform Infrared (FTIR) analysis of C3-HRP confirmed the immobilization of the enzyme.
View Article and Find Full Text PDFA novel colorimetric assay for the detection of urinary N, N-diacetylspermine, a known biomarker for colorectal cancer.
Anal Biochem
February 2025
Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2E9, Canada; Department of Computer Sciences, University of Alberta, Edmonton, AB, T6G 2E8, Canada; Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, T6G 1C9, Canada; Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H7, Canada. Electronic address:
Article Synopsis
- Urinary N, N-diacetylspermine (DAS) is a low-concentration biomarker for colorectal cancer, but current detection methods are complex and costly, creating a demand for simpler alternatives, especially for low-resource settings.
- This study introduces an efficient, inexpensive colorimetric assay using recombinant diacetylspermine oxidase (rDAS Ox) to oxidize DAS, which produces hydrogen peroxide and generates a pink color proportional to DAS levels in urine.
- A novel two-column ion exchange resin protocol was developed to remove interfering metabolites and concentrate DAS, enabling detection of as little as 1 μM DAS and demonstrating effective correlation with known DAS concentrations in urine samples.
MgAl-Layered Double Hydroxide-Coated Bio-Silica as an Adsorbent for Anionic Pollutants Removal: A Case Study of the Implementation of Sustainable Technologies.
Int J Mol Sci
November 2024
Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11120 Belgrade, Serbia.
The adsorption efficiency of Cr(VI) and anionic textile dyes onto MgAl-layered double hydroxides (LDHs) and MgAl-LDH coated on bio-silica (b-SiO) nanoparticles (MgAl-LDH@SiO) derived from waste rice husks was studied in this work. The material was characterized using field-emission scanning electron microscopy (FE-SEM/EDS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopic (XPS) techniques. The adsorption capacities of MgAl-LDH@SiO were increased by 12.
View Article and Find Full Text PDFA dye-decolorizing peroxidase from Vibrio cholerae can demetallate heme.
J Inorg Biochem
January 2025
Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo 060-8628, Japan.
Iron is an essential element for bacterial survival. Bacterial pathogens have therefore developed methods to obtain iron. Vibrio cholerae, the intestinal pathogen that causes cholera, utilizes heme as an iron source.
View Article and Find Full Text PDFBias-free glucose/O bio-photoelectrochemical system for multi-energy conversion and phenolic pollutant degradation.
Biosens Bioelectron
December 2024
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, PR China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, PR China. Electronic address:
Developing a multi-functional green energy device that propels sustainable energy development and concurrently purifies environmental pollutants offers an irresistibly compelling vision for a cleaner future. Herein, we reported a bias-free glucose/O bio-photoelectrochemical system (BPECS) for both energy conversion and phenolic pollutants degradation. Coupling a glucose dehydrogenase (GDH) modified self-assembled meso-tetrakis (4-carboxyphenyl)-porphyrin (SA-TCPP)-sensitized TiO biophotoanode for glucose oxidation and nitrogen/oxygen doped cobalt single-atom catalyst (CoNOC) cathode for two-electron oxygen reduction, both solar and biochemical energies were converted into electric power in BPECS with a maximum power density of 296.
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