Cell-free preparation of Clostridium sphenoides degraded the insecticide lindane, the gamma-isomer of 1,2,3,4,5,6-hexachlorocyclohexane, to the gamma-isomer of 3,4,5,6-tetrachloro-1-cyclohexene. The activity appeared to be associated with the membrane fraction and required reduced glutathione. The tetrachlorocy-clohexene intermediate was further metabolized by the membrane fraction to unknown substances.
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http://dx.doi.org/10.1128/aem.34.2.222-224.1977 | DOI Listing |
Int J Syst Evol Microbiol
January 2020
Department of Biology, University of Massachusetts, Amherst, Massachusetts, USA.
The genus belongs to the family However, many species with the genus name are found in different families and even crossing into a different phylum. Motivated by recently completed genome sequences, we propose the reclassification of two separate clades that include misclassified species which phylogenetically lie within the family , known for being benign members of gut microbiomes and for their plant-degrading capabilities. We use several phylogenetic and phylogenomic perspectives as well as phenotypic comparisons to gain insight into the evolutionary history of these taxa.
View Article and Find Full Text PDFExtremophiles
March 2019
CNRS/Universitè de Pau et des Pays de l'Adour/E2S, Institut des Sciences Analytiques et de Physicochimie pour l'Environnement et les Matériaux, Environmental Microbiology Group, UMR 5254, 64000, Pau, France.
Vapor steam vents are prevailing structures on geothermal sites in which local geochemical conditions allow the development of extremophilic microorganisms. We describe the structure of the prokaryotic community able to grow on the walls and rocks of such microecosystems in two terrestrial Mexican volcanoes: Paricutín (PI and PII samples) and its satellite Sapichu (S sample). The investigated samples showed similar diversity indices, with few dominant OTUs (abundance > 1%): 21, 16 and 23, respectively for PI, PII and S.
View Article and Find Full Text PDFMil Med
January 2017
Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910.
We report a case of successful treatment of chronic osteomyelitis (emanating from contaminated soil exposure) caused by Clostridium sphenoides, an organism infrequently identified as a cause of human infection and more saliently osteomyelitis (only 1 reported case in the literature). Additional impetus for reporting this case resides in the insights gained regarding pathogen identification exploiting sophisticated molecular platforms coupled to traditional microbial culture-based methods. The fastidious nature of cultivating anaerobic organisms required initial attempts at 16S rRNA sequencing to identify a Clostridium species (Clostridium celerecrescens).
View Article and Find Full Text PDFA fermentative, non-spore forming, motile, rod-shaped bacterium, designated strain MJ1(T), was isolated from an RDX contaminated aquifer at a live-fire training site in Northwest NJ, United States. On the basis of 16S rRNA gene sequencing and DNA base composition, strain MJ1(T) was assigned to the Firmicutes. The DNA G+C content was 42.
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