Cell-free preparation of Clostridium sphenoides degraded the insecticide lindane, the gamma-isomer of 1,2,3,4,5,6-hexachlorocyclohexane, to the gamma-isomer of 3,4,5,6-tetrachloro-1-cyclohexene. The activity appeared to be associated with the membrane fraction and required reduced glutathione. The tetrachlorocy-clohexene intermediate was further metabolized by the membrane fraction to unknown substances.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC242623PMC
http://dx.doi.org/10.1128/aem.34.2.222-224.1977DOI Listing

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