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Breaking the Myth of Enzymatic Azoreduction.
ACS Chem Biol
January 2025
Department of Chemistry, Johns Hopkins University, 3400 N. Charles St., Baltimore, Maryland 21218, United States.
Flavin-dependent azoreductases have been applied to a wide range of tasks from decolorizing numerous azo dyes to releasing azo-conjugated prodrugs. A general narrative reiterated in much of the literature suggests that this enzyme promotes sequential reduction of both the azo-containing substrate and its corresponding hydrazo product to release the aryl amine components while consuming two equivalents of NAD(P)H. Indeed, such aryl amines can be formed by incubation of certain azo compounds with azoreductases, but the nature of the substrates capable of this apparent azo bond lysis remained unknown.
View Article and Find Full Text PDFSugar signals pedal the cell cycle!
Front Plant Sci
March 2024
National Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, India.
Cell cycle involves the sequential and reiterative progression of important events leading to cell division. Progression through a specific phase of the cell cycle is under the control of various factors. Since the cell cycle in multicellular eukaryotes responds to multiple extracellular mitogenic cues, its study in higher forms of life becomes all the more important.
View Article and Find Full Text PDFSequential Mapping of Game Patterns in Men and Women Professional Padel Players.
Int J Sports Physiol Perform
May 2024
Sport Sciences Faculty, University of Granada, Granada, Spain.
Purpose: This study analyzed the sequences of actions in professional men and women padel players to identify common game patterns.
Methods: The sample comprised 17,557 stroke-by-stroke actions (N = 1640 rallies) of the championship World Padel Tour. Multistep Markov chains were used to calculate the conditional probabilities of occurrence of actions during the rally.
Formation of the brain by stem cell divisions of large neuroblasts in Oikopleura dioica, a simple chordate.
Dev Genes Evol
June 2023
Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka, 560-0043, Japan.
Stem cell division contributes to the generation of various cell types during animal development, especially a diverse pool of neural cells in the nervous system. One example is reiterated unequal stem cell divisions, in which a large stem cell undergoes a series of oriented unequal divisions to produce a chain of small daughter cells that differentiate. We show that reiterated unequal stem cell divisions are involved in the formation of the brain in simple chordate appendicularians (larvaceans).
View Article and Find Full Text PDFA new microfluidic platform for the highly reproducible preparation of non-viral gene delivery complexes.
Lab Chip
December 2022
Department of Chemistry, Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Milan, Italy.
Transfection describes the delivery of exogenous nucleic acids (NAs) to cells utilizing non-viral means. In the last few decades, scientists have been doing their utmost to design ever more effective transfection reagents. These are eventually mixed with NAs to give rise to gene delivery complexes, which must undergo characterization, testing, and further refinement through the sequential reiteration of these steps.
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