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Reactions of SleC, Its Structure and Inhibition in Mitigation of Spore Germination in .
J Am Chem Soc
January 2025
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, United States.
Spore germination in is initiated by a cascade of activities of several proteins that culminates in the activation of SleC, a cell-wall-processing enzyme. We report herein the details of the enzymatic activities of SleC by the use of synthetic peptidoglycan fragments and of spore sacculi. The reactions include the formation of 1,6-anhydromuramate─a hallmark of lytic transglycosylase activity─as well as a muramate hydrolytic product, both of which proceed through the same transient oxocarbenium species.
View Article and Find Full Text PDFHighly Efficient Biosynthesis of Rebaudioside M9 through Enzyme Screening and Structure-Guided Engineering.
J Agric Food Chem
January 2025
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, PR China.
Steviol glycosides (SGs) are highly valued for their sweetness, safety, and zero calories, but their bitter taste and low solubility limit their application. Modifying glycosyl units is a promising strategy to improve sensory qualities. In this study, we identified the enzyme UGT94E13 through phylogenetic analysis and enzyme screening, which catalyzes the glycosylation of rebaudioside M2 (Reb M2) at the C-13 position, producing the novel β-1,6--glycosylated product rebaudioside M9 (Reb M9).
View Article and Find Full Text PDFA review of the 's intervention mechanism and clinical application in ischemic stroke.
Front Pharmacol
January 2025
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Background: (BC), also named Niuhuang in Chinese, is utilized as a resuscitation drug in Traditional Chinese Medicine (TCM) for the treatment of neurological disorders. Ischemic stroke (IS) is a significant global public health issue that currently lacks safe and effective therapeutic drugs. Ongoing efforts are focused on identifying effective treatment strategies from Traditional, Complementary, and Integrative Medicine.
View Article and Find Full Text PDFBiocatalytic diversification of abietic acid in Streptomyces.
J Ind Microbiol Biotechnol
January 2025
Department of Chemistry, University of Florida, Gainesville, FL, USA.
Biocatalysis provides access to synthetically challenging molecules and commercially and pharmaceutically relevant natural product analogs while adhering to principles of green chemistry. Cytochromes P450 (P450s) are amongst the most superlative and versatile oxidative enzymes found in nature and are desired regio- and stereoselective biocatalysts, particularly for structurally complex hydrocarbon skeletons. We used 10 genome-sequenced Streptomyces strains, selected based on their preponderance of P450s, to biotransform the bioactive diterpenoid abietic acid.
View Article and Find Full Text PDFCofactor-directed co-immobilization of dual-enzyme on functionalized montmorillonite with enhanced catalytic performance.
Int J Biol Macromol
January 2025
School of Environment and Resource, Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education, Southwest University of Science and Technology, Mianyang, Sichuan 621010, China.
Recently, multi-enzyme cascade catalysis has attracted increasing attention due to the advantages of integrating multiple enzymes, few side reactions and high catalytic efficiency. Herein, a novel dual-enzyme cascade system (GOx-FMt-HRP) was developed through cofactor-directed orientational co-immobilization of glucose oxidase (GOx) and horseradish peroxidase (HRP) onto functional montmorillonite (FMt). The presented method realizes the reconstitution of cofactors and apo-enzymes (enzymes without cofactors), which enables enzymes to be immobilized in specific orientations on the support, thereby effectively reducing changes in their conformation.
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