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Interactions of elastin fibers with fibroblasts a time-lapse cinemicrographic study.
Pathol Biol (Paris)
September 1998
INSERM U460, Paris, France.
Adhesion of cells to the extracellular matrix is mediated by structural glycoproteins such as fibronectin and laminin, and also elastonectin, whose role is to ensure binding of elastin fibers to cells. Interactions between elastin fibers and human skin fibroblasts cultured in a Rose chamber were investigated by using cinemicrography to observe elastin fiber attachment, detachment, and displacement over a five-day period. Elastin fiber displacement over the cell layer resulted in aggregation, which was measured using morphometry.
View Article and Find Full Text PDFFusion of Leishmania amazonensis parasitophorous vacuoles with phagosomes containing zymosan particles: cinemicrographic and ultrastructural observations.
Braz J Med Biol Res
August 1996
Unite d'Immunoparasitologie, Institut Pasteur, Paris, France.
Studies on fixed preparations have shown that vacuoles containing zymosan (Z) particles internalized by infected macrophages can selectively fuse with the large parasitophorous vacuoles (PVs) that shelter Leishmania amazonensis. To examine the kinetics of vacuolar fusion in individual cells, particles were followed by time-lapse cinemicrography from their uptake to their entry in a PV. Newly formed Z-containing vacuoles moved centripetally and, if they contacted a PV, the two vacuoles remained closely apposed for variable, often extended, periods of time before they eventually fused.
View Article and Find Full Text PDFVisual versus cinemicrographic evaluation of human sperm motility and morphology.
Arch Androl
January 1988
Research Division, University of Medicine and Dentistry of New Jersey, School of Osteopathic Medicine, Camden 08103.
Ratings of human sperm motility by visual estimation through the microscope remain important measures of semen quality and of male fertility. More objective methods, including cinemicrography, time lapse photography, and videomicrography, are advocated. Subjective (visual) and objective (cinemicrographic) ratings of motility were compared.
View Article and Find Full Text PDFThe duration and variability of cell cycles in epithelial and fibroblast-like mammalian sister cells with different types of intercellular contacts were estimated using time-lapse cinemicrographic technique. To study a possible interrelation between cell cycles of the sister cells, one cell in each pair of sister cells was inactivated by selective UV microbeam irradiation at the beginning of its cell cycle. It is shown that this action may delay the cycle of the intact cell as well.
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