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Retention mechanism in slalom chromatography: Perspectives on the characterization of large DNA and RNA biopolymers in cell and gene therapy.
J Chromatogr A
January 2025
Waters Corporation, Instrument/Core Research/Fundamental, Milford, MA, 01757, USA. Electronic address:
Significant progress has been made in the last two decades in producing small (<2μm), high-purity, and low-adsorption particles, columns and system hardware, for ultra-high pressure liquid chromatography (UHPLC). Simultaneously, the recent rapid expansion of cell and gene therapies for treating diseases necessitates novel analytical technologies for analyzing large (>2 kbp) plasmid double-stranded (ds) DNA (which encodes for the in vitro transcription (IVT) of single-stranded (ss) mRNA therapeutics) and dsRNAs (related to IVT production impurities) biopolymers. In this context, slalom chromatography (SC), a retention mode co-discovered in 1988, is being revitalized using the most advanced column technologies for improved determination of the critical quality attributes (CQAs) of such new therapeutics.
View Article and Find Full Text PDFA Review of Sulfate Removal from Water Using Polymeric Membranes.
Membranes (Basel)
January 2025
Industrial Systems Engineering, Produced Water Treatment Laboratory, Faculty of Engineering and Applied Science, University of Regina, Regina, SK S4S 0A2, Canada.
Access to clean and reliable water has become a critical concern due to the global water crisis. High sulfate levels in drinking water raise health concerns for humans and animals and can cause serious corrosion in industrial systems. Sulfated waters represent a major challenge on the Canadian prairies, leading to many cattle deaths.
View Article and Find Full Text PDFCaustic recovery from caustic-containing polyethylene terephthalate (PET) washing wastewater generated during the recycling of plastic bottles.
Sci Rep
January 2025
Department of Mechanical and Industrial Engineering, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India.
To prevent water scarcity, wastewater must be discharged to the surface or groundwater after being treated. Another method is to reuse wastewater in some areas after treatment and evaluate it as much as possible. In this study, it is aimed to recover and reuse the caustic (sodium hydroxide, NaOH) used in the recycling of plastic bottles from polyethylene terephthalate (PET) washing wastewater.
View Article and Find Full Text PDFA Non-Centrifugation Method to Concentrate and Purify Extracellular Vesicles Using Superabsorbent Polymer Followed by Size Exclusion Chromatography.
J Extracell Vesicles
January 2025
Department of Internal Medicine and Clinical Nutrition, Krefting Research Centre, Institute of Medicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Extracellular vesicles (EVs) can be isolated and purified from cell cultures and biofluids using different methodologies. Here, we explored a novel EV isolation approach by combining superabsorbent polymers (SAP) in a dialysis membrane with size exclusion chromatography (SEC) to achieve high concentration and purity of EVs without the use of ultracentrifugation (UC). Suspension HEK293 cells transfected with CD63 coupled with Thermo Luciferase were used to quantify the EV yield and purity.
View Article and Find Full Text PDFPurification of mesenchymal stromal cell-derived small extracellular vesicles using ultrafiltration.
J Extracell Biol
January 2025
Institute of Biomedical Engineering, Department of Engineering Science University of Oxford Oxford UK.
Mesenchymal stromal cell-derived small extracellular vesicles (MSC-sEVs) are pivotal for the curative effects of mesenchymal stromal cells, but their translation into clinical products is hindered by the technical challenges of scaled production and purification. Ultrafiltration, a pressure-driven membrane separation method, is well known as an efficient, scalable, and cost-effective approach for bioseparation. However, there has been little study so far that comprehensively evaluates the potential application of ultrafiltration for scaled sEV isolation and purification.
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