The ability of cultured lymphoid, erythroid and fibroblastic cells for ligand-induced redistribution of their surface receptors proved to be lower than that of lymphocytes. The process of surface receptors redistribution can be inhibited by low temperature and sodium azide in all the cells under study. Pretreatment with trypsin increased the ability for redistribution of surface receptors in some types of cultured cells, but had no such effect on lymphocytes. These differences between lymphocytes and cultured cells seem to be due to differences in organization of the surface and contractile systems of the respective cells.
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Sci Rep
January 2025
Biological Engineering Program, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok, 10140, Thailand.
Nanobodies (Nbs) hold great potential to replace conventional antibodies in various biomedical applications. However, conventional methods for their discovery can be time-consuming and expensive. We have developed a reliable protein selection strategy that combines magnetic activated cell sorting (MACS)-based screening of yeast surface display (YSD) libraries and functional ligand-binding identification by Tat-based recognition of associating proteins (FLI-TRAP) to isolate antigen-specific Nbs from synthetic libraries.
View Article and Find Full Text PDFCell Commun Signal
January 2025
Dipartimento di Scienze della Vita e Sanità Pubblica, Università Cattolica del Sacro Cuore, Rome, Italy.
Background: Neuropilin-1 (NRP1) is a transmembrane protein involved in surface receptor complexes for a variety of extracellular signals. NRP1 expression in human cancers is associated with prominent angiogenesis and advanced progression stage. However, the molecular mechanisms underlying NRP1 activity in the tumor microenvironment remain unclear.
View Article and Find Full Text PDFJ Control Release
January 2025
Medicinal Materials Research Center, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea. Electronic address:
Receptor-mediated endocytosis plays a crucial role in the success of numerous therapies and remains central to advancing drug development. This process begins with ligand binding to specific receptors, triggering the internalization and intracellular trafficking of receptor-ligand complexes. These complexes are subsequently directed into distinct routes, either toward lysosomal degradation or recycling to the cell surface, with implications for therapeutic outcomes.
View Article and Find Full Text PDFPharmacol Res
January 2025
School of Pharmaceutical Sciences, University of Geneva, Rue Michel-Servet 1, 1211 Geneva 4, Switzerland; Institute of Pharmaceutical Sciences of Western Switzerland, Rue Michel-Servet 1, 1211 Geneva 4, Switzerland. Electronic address:
Osteoarthritis (OA) is the most affected joint disease worldwide, touching millions of people every year. It is caused by a progressive degeneration of articular cartilage, causing pain and limited mobility. Among the pathways involved in cartilage homeostasis, "LOX" proteins (referring to three distinct protein families, very often confused in the literature) play a prominent role.
View Article and Find Full Text PDFACS Nano
January 2025
School of Mechanical Engineering, Sichuan University, Chengdu 610065, China.
Natural skin receptors use ions as signal carriers, while most of the developed artificial tactile sensors utilize electrons as information carriers. To imitate the biological ionic sensing behavior, here, we present a kind of biomimetic, ionic, and fully passive mechanotransduction mechanism leveraging mechanical modulation of interfacial ionic p-n junction (IPNJ) through microchannels. Sensors based on this mechanism do not rely on an external power supply and can encode external tactile stimuli into highly analogous signal outputs to those of natural skin receptors, in terms of both signal type (i.
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