A disk method was developed for demonstrating penicillin tolerance in viridans streptococci. This was achieved by the substitution of the penicillin disk used for susceptibility testing by a disk containing penicillinase. After reincubation, penicillin-tolerant strains exhibited new growth in the area adjacent to this disk, providing a rapid screening test.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC272460PMC
http://dx.doi.org/10.1128/jcm.16.4.744-746.1982DOI Listing

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