The procedure for isolation of acylases from the fermentation broth filtrates of 3 actinomycetous cultures was developed with a yield of 72-97 per cent and 11-19- fold purification of the preparations. Comparative study of substrate specificity of acylase preparations showed that all of them possessed 2 types of the activity, i.e. penicillin acylase and aminoacylase cones. It was found that phenoxymethylpenicillin and acetylamino acids were the preferable substrates for revealing the hydrolytic activity of the acylases among beta-lactam antibiotics and acyl derivatives of amino acids, respectively.
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