In addition to the conventional methods for the identification of Enterobacteriaceae, enzymatic tests using chromogenic substrates have been proposed [1, 2, 5, 7, 8, 9, 10]. Many chromogenic and fluorogenic substrates are now available, and some of these have been employed for the determination of enzymatic profiles of Neisseria [3] and Enterobacteriaceae [7]. In this article, we report the activity of bacterial cultures of the genus Shigella on a new chromogenic substrate: chromozym PL.
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