AI Article Synopsis
- Significant changes in heart metabolism occur after alloxan injection in rats, affecting carbohydrate and lipid metabolism 48 hours post-injection.
- It appears that in vivo oxidation phosphorylation is disrupted in alloxanized rats, while normal metabolism can occur in well-oxygenated conditions in vitro.
- The study found significant reductions in energy compounds ATP and phosphocreatine, indicating impaired energy production, but insulin treatment normalized these compounds, suggesting oxygen delivery influences heart metabolism in diabetic conditions.
Article Abstract
Significant alterations in heart carbohydrate and lipid metabolism are present 48 h after intravenous injection of alloxan (60 mg/kg) in rats. It has been suggested that uncoupling of oxidative phosphorylation occurs in the alloxanized rat heart in vivo, whereas normal oxidative metabolism has been demonstrated in alloxan-diabetic rat hearts perfused in vitro under conditions of adequate oxygen delivery. We examined the hypothesis that high-energy phosphate metabolism might be adversely affected in the alloxan-diabetic rat heart in vivo. Phosphocreatine and ATP were reduced by 58 and 45%, respectively (P is less than 0.001). Also, oxygen-dissociation curves were shifted to the left by 4 mmHg, and the rate of oxygen release from blood was reduced by 21% (P is less than 0.01). Insulin administration normalized heart high-energy phosphate compounds. ATP production was accelerated in diabetic hearts perfused in vitro with a well-oxygenated buffer. These studies support the hypothesis that oxidative ATP production in the alloxan-diabetic rat heart is reduced and suggest that decreased oxygen delivery may have a regulatory role in the oxidative metabolism of the diabetic rat heart.
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| http://dx.doi.org/10.1152/ajplegacy.1976.230.6.1744 | DOI Listing |
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