AI Article Synopsis
- Mitochondria that are uncoupled due to aging or freeze-thaw treatments can produce ATP from ADP and Pi when there is a rapid increase in external pH, but not during a decrease.
- The optimal ATP production is around 2.5 ATP molecules for each electron transport chain when the pH increase is over 0.7 units, and the pH crosses the range of 8.1 to 8.3.
- This ATP synthesis triggered by a pH jump is fully blocked by the compound oligomycin.
Article Abstract
Mitochondria, uncoupled by aging or by freeze-thaw treatment, are able to synthesize ATP from ADP and Pi after a fast increase (but not decrease) in the external pH. The maximal ATP yield (approx. 2.5 ATP molecules/electron-transport chain per pH jump) can be obtained under the following conditions: (1) the pH change during the jump must exceed 0.7 pH units; (2) in the course of this change, the pH of the mitochondrial suspension must cross the pH 8.1-8.3 value. This pH-jump-induced ATP synthesis is completely inhibited by oligomycin.
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| http://dx.doi.org/10.1016/0005-2728(82)90132-3 | DOI Listing |
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Article Synopsis
- Mitochondria that are uncoupled due to aging or freeze-thaw treatments can produce ATP from ADP and Pi when there is a rapid increase in external pH, but not during a decrease.
- The optimal ATP production is around 2.5 ATP molecules for each electron transport chain when the pH increase is over 0.7 units, and the pH crosses the range of 8.1 to 8.3.
- This ATP synthesis triggered by a pH jump is fully blocked by the compound oligomycin.
The kinetic properties of pH jump-induced phosphorylation in thylakoidal membranes of spinach chloroplasts were investigated, and the following results were obtained. 1. The pH jump-induced P incorporation proceeded linearly with time for at least 2 sec after the start of the reaction.
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