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Development, Optimization, and Evaluation of Rutin-Loaded Liposomes in the Management of Rheumatoid Arthritis.
Curr Drug Deliv
January 2025
Department of Pharmaceutical Sciences, Gurugram University, Gurugram - 122018, India.
Background: Rheumatoid arthritis is a chronic autoimmune disease, progressively distinctive via cartilage destruction, auto-antibody production, severe joint pain, and synovial inflammation. Nanotechnology represents one of the utmost promising scientific technologies of the 21st century. Nanocarriers could be the key to unlocking its potential by encapsulating Rutin in targeted drug delivery systems, potentially for targeted Rheumatoid arthritis therapy.
View Article and Find Full Text PDFHeterologous expression of calcium-independent mesophilic α-amylase from Priestia megaterium: Immobilization on genipin-modified multi-walled carbon nanotubes and silica supports to enhance thermostability and catalytic activity.
Bioorg Chem
January 2025
Department of Bioengineering, Gebze Technical University, 41400, Gebze, Kocaeli, Türkiye; BAUZYME Biotechnology Co., Gebze Technical University Technopark, 41400, Gebze, Kocaeli, Türkiye. Electronic address:
α-Amylases, constituting a significant share of the enzyme market, are mainly synthesized by the genus Bacillus. Enzymes tailored for specific industrial applications are needed to meet the growing demand across a range of industries, and thus finding new amylases and optimizing the ones that already exist are extremely important. This study reports the successful expression, characterization and immobilization of P.
View Article and Find Full Text PDFOptimized microwave-assisted extraction and characterization of spray dried Luffa aegyptiaca nanomucilage: Physicochemical properties, biological activities, and anticancer efficacy against MCF-7 human breast cancer cells.
Int J Biol Macromol
January 2025
Department of Food Technology and Nutrition, Lovely Professional University, Phagwara 144411, India. Electronic address:
Microwave-assisted extraction conditions were optimized using response surface methodology to evaluate the effects of extraction parameters on the yield and carbohydrate content of Luffa aegyptiaca mucilage. Extraction at 540 W for 2 min with a 1:20 (g/mL) was determined as the optimal parameter, resulting in a maximum yield of 5.90 % (w/w) with 63 % carbohydrate content consisting of glucose, galactose, maltose, mannose, and galacturonic acid, with structural linkages of β (1 → 4) and β (1 → 6) glycosidic bonds.
View Article and Find Full Text PDFProtein thermal stability in the undergraduate biochemistry laboratory: Exploring protein thermal stability with yeast alcohol dehydrogenase.
Biochem Mol Biol Educ
January 2025
Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, USA.
We created a novel laboratory experience where undergraduate students explore the techniques used to study protein misfolding, unfolding, and aggregation. Despite the importance of protein misfolding and aggregation diseases, protein unfolding is not typically explored in undergraduate biochemistry laboratory classes. Yeast alcohol dehydrogenase (YADH) is used in the undergraduate biochemistry laboratory course at Miami University as the model system to explore protein overexpression and purification, bioinformatics, and enzyme characterization.
View Article and Find Full Text PDFImpact of thermal denaturation on renal volume reduction after partial nephrectomy using soft coagulation hemostasis.
Sci Rep
January 2025
Department of Urology, NTT Medical Center Tokyo, 5-9-22 Higashigotanda, Shinagawa-ku, Tokyo, 141-8625, Japan.
Partial nephrectomy has become the gold standard treatment for small renal masses. This study aimed to assess the impact of soft coagulation hemostasis on parenchymal volume reduction of the operated kidney after an open partial nephrectomy. We retrospectively reviewed 94 patients with small renal tumors who underwent open partial nephrectomy with soft-coagulation hemostasis at our institution.
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