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Enhancing RT-PCR Throughput and Sensitivity through Large-Scale Sample Pooling Using a Nano-Hybrid Membrane.
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Department of Electrical Engineering, Kwangwoon University, Seoul, 01897, Republic of Korea.
During the pandemic surge, including SARS-CoV-2 and influenza, pooling samples emerged as an efficient strategy to identify infected individuals in large groups. While pooling enhances RT-PCR throughput, reducing costs and resources, it dilutes positive samples with negative ones, lowering sensitivity and increasing false negatives. This study proposes a new method to address the trade-off between pool sizes and RT-PCR accuracy.
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Aerosol Technology Laboratory, Biological and Agricultural Engineering Department, Texas A&M University, College Station, TX, 77843, USA.
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Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China.
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Department of Molecular Biology & Bioinformatics, Tripura University (A Central University), Suryamaninagar, 799022, Tripura, India. Electronic address:
Biofilm formation, extracellular substance synthesis, and virulence factor production all have a major impact on drug tolerance and infection propagation caused by Staphylococcus aureus. Flavonoid compounds have been explored as potential solutions to enhance antibiotic efficacy against the biofilm formation of pathogenic microbes. Quercetin (QER) has previously demonstrated antibacterial and antibiofilm properties.
View Article and Find Full Text PDFGymnema saponin-induced lipid flip-flop identifies rigid membrane phenotype of methicillin resistant S. aureus and enhances it's antibiotic susceptibility.
Arch Biochem Biophys
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Department of Biotechnology, Maharaja Sriram Chandra Bhanjadeo University, (Erstwhile: North Orissa University), Baripada, Odisha, 757003, India. Electronic address:
Our previous study revealed that lipid flip-flop inducing phytochemicals from Gymnema sylvestre increase membrane permeability of antimicrobials in S. aureus. However, their lipid flipping and membrane permeabilizing effect on methicillin resistant S.
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