Tubulin was tyrosinated in slices and in extracts of brain of rats of 3, 25, and 120 days of age by successive incorporation of [14C]tyrosine and [3H]tyrosine, respectively. The release of the incorporated amino acid was measured by using tubulinyl-tyrosine carboxypeptidase, carboxypeptidase A, and tubulin-tyrosine ligase. With the carboxypeptidases no differences in either the rates or the extents of the release of tyrosine between these two differently labeled tubulins were found. Differences were found when the detyrosination was catalyzed by the ligase and these were attributed to a higher inactivation of tubulin labeled in slices than of that labeled in extracts.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1471-4159.1982.tb10860.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Epothilone D on Social Defeat Stress-induced Changes in Microtubule-related and Endoplasmic Reticulum Stress Protein Expression.
Clin Psychopharmacol Neurosci
February 2025
Department of Psychiatry, Jeonbuk National University Medical School, Jeonju, Korea.
Objective: Epothilone D (EpoD), microtubule (MT) stabilizing agent, demonstrated promising results in the animal models of Alzheimer's disease, Parkinson's disease and schizophrenia. The present study sought to investigate preventive effects of EpoD on altered changes of MT related proteins and endoplasmic reticulum (ER) stress proteins induced by social defeat stress (SDS).
Methods: We measured protein expression levels of α-tubulin and its post-translational modifications, MT-associated protein 2, stathmin1 and 2 with their phosphorylated forms, and ER stress markers, 78-kDa glucose-regulated protein (GRP-78) and CCAAT/enhancer binding protein (C/EBP)-homologous protein (CHOP) in the prefrontal cortex (PFC) and hippocampus (HIP) of C57BL/6J strain mice treated with EpoD (2 mg/kg) or its vehicle, dimethylsulfoxide (DMSO), and exposed to SDS.
Abscisic Acid, Microtubules and Phospholipase D-Solving a Cellular Bermuda Triangle.
Int J Mol Sci
December 2024
Molecular Cell Biology, Joseph Gottlieb Kölreuter Institute for Plant Sciences, Karlsruhe Institute of Technology, Fritz-Haber-Weg 4, 76131 Karlsruhe, Germany.
Rice plants are important food crops that are sensitive to cold stress. Microtubules (MTs) are highly associated with plant response to cold stress. The exogenous application of abscisic acid (ABA) can transiently induce the cold stability of microtubules.
View Article and Find Full Text PDFα-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.
Nat Commun
November 2024
i3S-Institute for Research and Innovation in Health, University of Porto, Rua Alfredo Allen 208, 4200-135, Porto, Portugal.
Post-translational cycles of α-tubulin detyrosination and tyrosination generate microtubule diversity, the cellular functions of which remain largely unknown. Here we show that α-tubulin detyrosination regulates kinetochore-microtubule attachments to ensure normal chromosome oscillations and timely anaphase onset during mitosis. Remarkably, detyrosinated α-tubulin levels near kinetochore microtubule plus-ends depend on the direction of chromosome motion during metaphase.
View Article and Find Full Text PDFChronic Activation of Tubulin Tyrosination Improves Heart Function.
Circ Res
October 2024
Department of Experimental Pharmacology and Toxicology (N.P., B.G., E.K., G.M., S.S., L.C.), University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Article Synopsis
- - Hypertrophic cardiomyopathy (HCM) is a common genetic heart disorder linked to sarcomere gene mutations, resulting in left ventricular thickening and diastolic dysfunction; new research emphasizes the importance of microtubule alterations in heart failure.
- - The study explored the effects of increasing tubulin tyrosination via adeno-associated virus transfer in various models, including HCM human cardiomyocytes and specific mouse models, revealing that this approach improved heart function by reducing harmful microtubule modifications and enhancing contractility.
- - Results indicated that enhancing tubulin tyrosination led to better heart function metrics such as contractility and cardiac output in both human and mouse models, while also suggesting potential benefits of targeting the micro
Protofilament-specific nanopatterns of tubulin post-translational modifications regulate the mechanics of ciliary beating.
Curr Biol
October 2024
Human Technopole, V.le Rita Levi-Montalcini 1, Milan 20157, Italy. Electronic address:
Article Synopsis
- Controlling ciliary beating is crucial for movement and signaling in eukaryotic cells, relying on the organization of axonemal proteins on microtubules.
- Tubulin post-translational modifications (PTMs) like glycylation and polyglutamylation play key roles in this process, creating specific nanopatterns that help regulate ciliary function.
- The study highlights the existence of a ciliary tubulin "nanocode," suggesting that researching these modifications at a high resolution could enhance our understanding of their roles in other cellular structures.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!