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Ultra pure high molecular weight DNA from soil for Nanopore shotgun metagenomics and metabarcoding sequencing.
MethodsX
June 2025
IRD, UMR Eco&Sols, INRAE, CIRAD, Institut Agro, Université Montpellier, Montpellier, France.
Soil microbes are among the most abundant and diverse organisms on Earth but remain poorly characterized. New technologies have made possible to sequence the DNA of uncultivated microorganisms in soil and other complex ecosystems. Genome assembly is crucial for understanding their functional potential.
View Article and Find Full Text PDFFirst identification report for amino acid composition of red algae spp. obtained from Central Java, Indonesia.
BioTechnologia (Pozn)
December 2024
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia.
is a genus of red algae found mainly in Asia. Various species of are distributed throughout Indonesia's marine waters, especially in coastal areas, and are cultivated for research and economic purposes. mainly consists of polysaccharides and pigments, which have hindered protein identification.
View Article and Find Full Text PDFPositive real-time PCR in pneumococcal meningitis 12 hours after initiation of antibiotic therapy - case report.
BMC Neurol
January 2025
Department of Neurology, LMU University Hospital, LMU Munich, Marchioninistrasse 15, 81377, Munich, Germany.
Background: Purulent meningitis poses a significant clinical challenge with high mortality. We present the case of a 54-year-old female transferred to our emergency department with suspected bacterial meningitis, later diagnosed as an Austrian syndrome.
Case Presentation: The patient exhibited subacute somnolence, severe headache, nausea and fever.
Isolation, characterization and therapeutic efficacy of lytic bacteriophage ZK22 against Salmonella Typhimurium in mice.
BMC Microbiol
January 2025
Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, Guangdong, China.
Background: Salmonella enterica serovar Typhimurium is one of the most common serovars of Salmonella associated with clinical cases. It not only leads to diarrhea and mortality raised in livestock and poultry farming, but also poses a risk to food safety.
Results: In this study, a lytic bacteriophage named ZK22 was isolated and identified from sewage.
The gut microbiota predicts and time-restricted feeding delays experimental colitis.
Gut Microbes
December 2025
Department of Microbiome Research and Applied Bioinformatics, Institute for Nutritional Sciences, University of Hohenheim, Stuttgart, Germany.
The etiology of inflammatory bowel disease (IBD) remains unclear, treatment options unsatisfactory and disease development difficult to predict for individual patients. Dysbiosis of the gastrointestinal microbiota and disruption of the biological clock have been implicated and studied as diagnostic and therapeutic targets. Here, we examine the relationship of IBD to biological clock and gut microbiota by using the IL-10 deficient () mouse model for microbiota-dependent spontaneous colitis in combination with altered (4 h/4 h) light/dark cycles to disrupt and time-restricted feeding (TRF) to restore circadian rhythmicity.
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