A fully automated blood grouping machine, that has recently been introduced was evaluated. The instrument incorporates bar-coded sample identification and a laser scanning device to eliminate potential sample/result errors. A microprocessor interprets results, thus eliminating subjective interpretation. Ninety-seven percent of the 17,549 samples tested were correctly grouped. One-third of the initially rejected samples were correctly grouped upon retesting. The laser scanner failed to read 239 labels (1.6%). Of the original samples tested, 72 were Du positive as detected by manual methods and the automated grouper recognized 33 percent of them as Rh positive. By reducing the percent light transmission affecting Rh discrimination, it was possible to increase the percentage recognition of Du variant samples but it did not detect all. The apparatus is fitted with small diameter tubing and reagent consumption was 30 to 35 percent less than with previously available similar machines. Additional reagent savings were realized if diluted manual reagents were used. Our evaluation of the instrument has shown it to be highly accurate and reliable.
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http://dx.doi.org/10.1046/j.1537-2995.1982.22482251220.x | DOI Listing |
Analyst
January 2025
Faculty of Physics, M.V. Lomonosov Moscow State University, 1-2 Leninskie Gory, Moscow, 119991, Russia.
The issue of variability introduced into blood plasma and serum analysis by preanalytical procedures is the major obstacle to obtaining accurate and reproducible results. While the question of how to overcome this issue has been discussed in biochemical detection of analytes and omics technologies, its relevance to the field of optical spectroscopy remains mostly unexplored. In this work, we evaluated the freeze-thaw cycle (FTC)-induced alternations in blood serum optical properties by means of autofluorescence and Raman spectroscopy, including surface-enhanced Raman spectroscopy (SERS).
View Article and Find Full Text PDFFluids Barriers CNS
January 2025
Neurology 5 - Neuropathology Unit, Fondazione IRCCS - Istituto Neurologico Carlo Besta, Via Celoria 11, Milan, 20133, Italy.
Background: The approval of new disease-modifying therapies by the U.S. Food and Drug Administration and the European Medicine Agency makes it necessary to optimize non-invasive and cost-effective tools for the identification of subjects at-risk of developing Alzheimer's Disease (AD).
View Article and Find Full Text PDFTransfus Apher Sci
January 2025
ICMR-National Institute of Immunohaematology (NIIH), 13th Floor, K.E.M Hospital campus, Parel, Mumbai 400012, India. Electronic address:
This case report presents first case of RHD*weak D type 9 in a 38-year-old Indian patient with severe osteoarthritis of the left hip joint scheduled for total hip replacement surgery. During routine blood grouping, an unexpected weak reaction with anti-D was observed. Serological characterization using an extended partial D typing kit characterized the variant as DV.
View Article and Find Full Text PDFActa Ophthalmol
January 2025
Optics, Optometry and Vision Sciences, University of Valencia, Burjassot, Spain.
Aims/purpose: This study aims to assess whether changes in intraocular pressure (IOP) occur after a blood draw and quantify these changes' magnitude in a group of patients over 60 years old.
Methods: We analysed data from the right eye of 54 subjects, with a mean ( SD) age of 68 4 years. The measurements were conducted in the morning while fasting.
Acta Ophthalmol
January 2025
Optics, Optometry and Vision Sciences, University of Valencia, Burjassot, Spain.
Aims/purpose: This study aims to assess whether changes in intraocular pressure (IOP) occur after a blood draw and quantify these changes' magnitude in a group of patients over 60 years old.
Methods: We analysed data from the right eye of 54 subjects, with a mean ( SD) age of 68 4 years. The measurements were conducted in the morning while fasting.
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