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Interspecies synergistic interactions mediated by cofactor exchange enhance stress tolerance by inducing biofilm formation.
mSystems
September 2024
MOE Laboratory of Biosystem Homeostasis and Protection, College of Life Sciences, Zhejiang University, Hangzhou, China.
Unlabelled: Metabolic exchange plays a crucial role in shaping microbial community interactions and functions, including the exchange of small molecules such as cofactors. Cofactors are fundamental to enzyme catalytic activities; however, the role of cofactors in microbial stress tolerance is unclear. Here, we constructed a synergistic consortium containing two strains that could efficiently mineralize di-(2-ethylhexyl) phthalate under hyperosmotic stress.
View Article and Find Full Text PDFBehavioural and physiological impacts of low salinity on the sea urchin Echinus esculentus.
J Exp Biol
January 2024
British Antarctic Survey, Natural Environment Research Council, Cambridge CB3 0ET, UK.
Article Synopsis
- Reduced seawater salinity significantly affects the physiology of the European sea urchin, Echinus esculentus, with short-term exposure showing a direct correlation between lower salinity, oxygen consumption, and survival rates.
- Long-term exposure at 21‰ over 25 days led to increased oxygen consumption but decreased feeding and activity levels compared to normal salinity, while 26‰ showed acclimation with similar metrics to control values.
- The study highlights the sea urchin's phenotypic plasticity at around 26‰ salinity for acclimation, but notes that lower levels like 21‰ could restrict its habitat in areas with high freshwater influx.
Strikingly conserved gene expression changes of polyamine regulating enzymes among various forms of acute and chronic kidney injury.
Kidney Int
July 2023
Institute of Translational Physiology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany. Electronic address:
The polyamines spermidine and spermine and their common precursor molecule putrescine are involved in tissue injury and repair. Here, we test the hypothesis that impaired polyamine homeostasis contributes to various kidney pathologies in mice during experimental models of ischemia-reperfusion, transplantation, rhabdomyolysis, cyclosporine treatment, arterial hypertension, diabetes, unilateral ureteral obstruction, high oxalate feeding, and adenine-induced injuries. We found a remarkably similar pattern in most kidney pathologies with reduced expression of enzymes involved in polyamine synthesis together with increased expression of polyamine degrading enzymes.
View Article and Find Full Text PDFHyaluronic Acid 35 kDa Protects against a Hyperosmotic, Formula Feeding Model of Necrotizing Enterocolitis.
Nutrients
April 2022
Department of Pediatrics, Division of Neonatology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Necrotizing enterocolitis (NEC), an inflammatory disease of the intestine, is a common gastrointestinal emergency among preterm infants. Intestinal barrier dysfunction, hyperactivation of the premature immune system, and dysbiosis are thought to play major roles in the disease. Human milk (HM) is protective, but the mechanisms underpinning formula feeding as a risk factor in the development of NEC are incompletely understood.
View Article and Find Full Text PDFRecombinase-mediated cassette exchange-based screening of a CRISPR/Cas9 library for enhanced recombinant protein production in human embryonic kidney cells: Improving resistance to hyperosmotic stress.
Metab Eng
July 2022
Department of Biological Sciences, KAIST, Daejeon, 34141, Republic of Korea. Electronic address:
Targeted engineering of mammalian cells has been widely attempted to ensure the efficient production of therapeutic proteins with proper quality during bioprocesses. However, the identification of novel targets for cell engineering is labor-intensive and has not yet been fully substantiated. Here, we established a CRISPR/Cas9 library screening platform in human embryonic kidney (HEK293) cells based on guide RNA integration mediated by recombinase-mediated cassette exchange (RMCE) to interrogate gene function in a high-throughput manner.
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