The previously described thin-layer chromatography procedure (Brennan et al., J. Clin. Microbiol. 8:374-379, 1978) for identifying serovars of the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex, based on their specific C-mycoside glycopeptidolipid antigens, has now been extended to all 31 known serovars. Photographs of the characteristic pattern of the glycopeptidolipids are presented. Although the procedure is fraught with the difficulties inherent to comparative thin-layer chromatography, it is particularly suitable for the screening of isolates which are not amenable to seroagglutination, such as those which autoagglutinate or for which antisera are not presently available. In this way it was possible to tell whether isolates were of the M. avium-M. intracellulare-M. scrofulaceum complex and whether or not they had previously been recognized. Knowledge of the chemical features of the typing antigens nontuberculous mycobacteria other than M. avium-M. intracellulare-M. scrofulaceum strains also enables us to develop thin-layer chromatography systems for the identification of M. kansasii, M. szulgai, members of the M. gordonae complex, M. terrae, M. xenopi, and M. gastri.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC272116 | PMC |
| http://dx.doi.org/10.1128/jcm.15.3.447-455.1982 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preclinical evaluation of zirconium-89 labeled anti-Trop2 antibody-drug conjugate (Trodelvy) for imaging in gastric cancer and triple-negative breast cancer.
Eur J Nucl Med Mol Imaging
January 2025
Departments of Radiology and Medical Physics, University of Wisconsin - Madison, Madison, WI, 53705, USA.
Purpose: Trophoblast cell-surface antigen 2 (Trop2) is overexpressed in various solid tumors and contributes to tumor progression, while its expression remains low in normal tissues. Trop2-targeting antibody-drug conjugate (ADC), sacituzumab govitecan-hziy (Trodelvy), has shown efficacy in targeting this antigen. Leveraging the enhanced specificity of ADCs, we conducted the first immunoPET imaging study of Trop2 expression in gastric cancer (GC) and triple-negative breast cancer (TNBC) models using Zr-labeled Trodelvy ([Zr]Zr-DFO-Trodelvy).
View Article and Find Full Text PDFDevelopment of a novel molecular probe for visualizing mesothelin on the tumor via positron emission tomography.
Eur J Nucl Med Mol Imaging
January 2025
Institute of Radiation Medicine, Fudan University, Xietu Road 2094, Shanghai, 200032, China.
Objectives: Mesothelin (MSLN) is an antigen that is overexpressed in various cancers, and its interaction with tumor-associated cancer antigen 125 plays a multifaceted role in tumor metastasis. The serum MSLN expression level can be detected using enzyme-linked immunosorbent assay; however, non-invasive visualization of its expression at the tumor site is currently lacking. Therefore, the aim of this study was to develop a molecular probe for imaging MSLN expression through positron emission tomography (PET).
View Article and Find Full Text PDFIsolation of AP1, a rumen bacterium that forms 10,12-conjugated linoleic acid.
JDS Commun
January 2025
Department of Animal Science, University of California, Davis, Davis, CA 95168.
Some microbes in the rumen form 10,12 (10,12)-conjugated linoleic acid (CLA), a fatty acid that depresses synthesis of milk fat in dairy cattle and other lactating animals. Despite their importance to milk fat depression, the microbes responsible have been difficult to identify, and no laboratory strain is currently available for study. Here we describe the isolation of AP1, a bacterium that forms 10,12-CLA at fast rates.
View Article and Find Full Text PDFEvaluating the Effectiveness of a Novel Pongamia pinnata Derived Herbal Mouth-Dissolving Film for Treating Oral Disorders and Evaluating Its Anticancer Properties.
Cell Biochem Funct
February 2025
Department of Life Sciences, Hemchandracharya North Gujarat University, Patan, India.
The present study aimed to optimize a mouth-dissolving film (MDF) made from Pongamia pinnata stem bark extract to increase patient compliance and accelerate oral disease therapy. Several stem bark extracts were prepared, and karanjin was used as an herbal marker for the extracts. The ethanolic extract showed the maximum yield (12.
View Article and Find Full Text PDFThin Layer Chromatography Goes Ultrasmall to Assay Sphingosine Kinase Activation in Single Primary Leukemic Cells.
Anal Chem
January 2025
Department of Bioengineering, University of Washington, SeattleWashington98195, United States.
Cell-to-cell heterogeneity in lipid signaling underlies variations in response and recurrence for many cancers, including leukemias. A highly parallel, miniaturized thin-layer chromatographic platform capable of assaying single cells was developed. Ultrasmall volumes (50 pL) of standard fluorescent lipids were separated with excellent repeatability, reproducibility, and limits of detection.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!