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DNAzyme approach for simultaneous mRNA cap and poly(A) tail length analysis: A one-step method to multiple quality attributes.
J Pharm Biomed Anal
January 2025
Analytical Research and Development, Merck & Co., Inc., Rahway, NJ 07065, United States. Electronic address:
The dynamic landscape of mRNA technology highlights the need for innovative quality control (QC) strategies. In this study, we described an efficient one-step digestion approach for concurrent generation of 5'- and 3'-end fragments, enabling simultaneous mRNA capping and poly(A) tail analysis. Tailored 10-23-type DNAzymes, designed from 5'- and 3'-Untranslated Regions (UTRs), selectively cleaved mRNA to release both the 5'-Capped or uncapped short fragments and 3'-Poly(A) tail cleavage products.
View Article and Find Full Text PDFHigh-sensitivity flexible electrochemical sensor for real-time multi-analyte sweat analysis.
Talanta
January 2025
School of Material Science and Engineering, Shanghai University of Engineering Science, Shanghai, 201620, PR China. Electronic address:
Flexible sweat sensors play a crucial role in health monitoring and disease prevention by enabling real-time, non-invasive assessment of human physiological conditions. Sweat contains a variety of biomarkers, offering valuable insights into an individual's health status. In this study, we developed an advanced flexible electrochemical sensor featuring reduced graphene oxide (rGO)-based electrodes, modified with a composite material comprising nitrogen and sulfur co-doped holey graphene (HG) and MXene, with in-situ-grown TiO nanoparticles on the MXene.
View Article and Find Full Text PDFConfirming the validity and reliability of the Placement Evaluation Tool to evaluate midwifery practice placements (PET-Midwifery).
Midwifery
January 2025
Health Innovation and Transformation Centre, Federation University Australia, Clyde Rd Berwick, Victoria, 3806, Australia. Electronic address:
Problem: In Australia, program accreditation requirements include that education providers monitor and evaluate teaching and learning environments and provide evidence of outcomes being used to inform program quality improvement. Yet, closing this loop has proven challenging.
Background: The Australian National Placement Evaluation Centre (NPEC) functions to measure the quality of placements through student evaluations.
Construction and initial validations of the major decision-making self-efficacy scale (MDMSES) for Chinese high school students.
BMC Psychol
January 2025
School of Mental Health and Psychological Sciences, Anhui Medical University, Hefei, China.
Background: Major decision-making self-efficacy (MDMSE) is an important indicator of students' ability to make effective decisions in specialty selection. It has implications for students' personal growth and career counselling interventions. While the previous MDMSES has been widely used in the context of China's New College Entrance Examination reform, the increased choice of majors and advancement of career planning necessitate a new scale to assess high school students' MDMSE levels.
View Article and Find Full Text PDFPsychometric Evidence and Measurement Invariance by College-Going Status for the Inventory of the Dimensions of Emerging Adulthood (IDEA).
J Pers Assess
January 2025
Department of Counselor Education and School Psychology, Duquesne University.
Numerous versions exist of the Inventory of the Dimensions of Emerging Adulthood (IDEA), which is used to assess normative markers of development in the 18-29 age range. However, their comparative psychometric properties have not been adequately explored, and samples including non-college-going emerging adults are rare. In Study 1, confirmatory factor analyses using survey data from 429 college-going and non-college-going emerging adults (52% female, 44% non-white) showed the IDEA-Short Form (IDEA-SF) to be the most psychometrically and conceptually sound version, demonstrating good fit for the five-factor model proposed by Arnett's (2004) theory of emerging adulthood; initial evidence for concurrent validity of the IDEA-SF scores was also presented.
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