The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.
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