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Stabilisation of Na,K-ATPase structure by the cardiotonic steroid ouabain.
Biochem Biophys Res Commun
May 2013
Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK.
Cardiotonic steroids such as ouabain bind with high affinity to the membrane-bound cation-transporting P-type Na,K-ATPase, leading to complete inhibition of the enzyme. Using synchrotron radiation circular dichroism spectroscopy we show that the enzyme-ouabain complex is less susceptible to thermal denaturation (unfolding) than the ouabain-free enzyme, and this protection is observed with Na,K-ATPase purified from pig kidney as well as from shark rectal glands. It is also shown that detergent-solubilised preparations of Na,K-ATPase are stabilised by ouabain, which could account for the successful crystallisation of Na,K-ATPase in the ouabain-bound form.
View Article and Find Full Text PDFDeath of ouabain-treated renal epithelial cells: evidence for p38 MAPK-mediated Na (i) (+) /K (i) (+) -independent signaling.
Apoptosis
November 2009
Centre de recherche, Centre hospitalier de l'Université de Montréal, QC, Canada.
Recent studies demonstrate that cytotoxic actions of ouabain and other cardiotonic steroids (CTS) on renal epithelial cells (REC) are triggered by their interaction with the Na(+),K(+)-ATPase alpha-subunit but not the result of inhibition of Na(+),K(+)-ATPase-mediated ion fluxes and inversion of the [Na(+)](i)/[K(+)](i) ratio. This study examined the role of mitogen-activated protein kinases (MAPK) in the death of ouabain-treated REC. Exposure of C7-MDCK cells that resembled principal cells from canine kidney to 3 microM ouabain led to phosphorylation of p38 without significant impact on phosphorylation of ERK and JNK MAPK.
View Article and Find Full Text PDFRectal water absorption in seawater-adapted Japanese eel Anguilla japonica.
Comp Biochem Physiol A Mol Integr Physiol
December 2008
Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan.
Marine teleosts drink large amounts of seawater to compensate for continuous osmotic water loss. We investigated a possible significant role of the rectum in water absorption in seawater-adapted eel. In rectal sacs filled with balanced salt solution (BSS) and incubated in isotonic BSS, water absorption was greater in seawater-adapted eel than in freshwater eel.
View Article and Find Full Text PDFHeterogeneity of Na+/K+-ATPase from rectal gland of Squalus acanthias is not due to alpha isoform diversity.
Pflugers Arch
March 1999
Department of Physiology, Aarhus University, Ole Worms Allé 160, DK-8000 Arhus C, Denmark.
Purified Na+/K+-ATPase (EC 3.6.1.
View Article and Find Full Text PDFRole of guanylyl cyclase receptors for CNP in salt secretion by shark rectal gland.
Am J Physiol
October 1997
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA.
The role of C-type natriuretic peptide (CNP) and its guanylyl cyclase-linked receptors in mediating salt secretion by the rectal gland of the spiny dogfish shark (Squalus acanthias) was investigated using HS-142-1, a competitive inhibitor of the binding of natriuretic peptides to their guanylyl cyclase receptors. CNP binds to receptors and activates guanylyl cyclase in rectal gland membranes in a way that is inhibited by HS-142-1. Guanylyl cyclase activation in rectal gland membranes is far more sensitive to CNP than to atrial natriuretic peptide, whereas the reverse is true for membranes derived from mammalian (rabbit) renal collecting duct cells.
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