AI Article Synopsis
- The study successfully adapted a method for measuring lipase in human serum using dimercaprol (BAL) tributyrate-5,5'-dithiobis(2-nitrobenzoic acid) for two types of analyzers: a discrete analyzer (TBA 880) and a continuous-flow analyzer (AutoAnalyzer, Type II).
- Both methods utilized BAL-tributyrate as the substrate alongside serum esterase inhibitors and a chromogenic reagent, allowing for effective lipase activity measurement in patients with pancreatic diseases.
- The correlation of results from these automated methods with traditional manual methods and Kaplan's radioassay was very strong, with correlation coefficients above 0.95 and low variation (less than 8%), indicating high reliability
Article Abstract
We successfully adapted the dimercaprol (BAL) tributyrate-5,5'-dithiobis(2-nitrobenzoic acid) method (J. Biochem. 81: 361, 1977) for assay of lipase in human serum to a discrete analyzer (the TBA 880) (I) or a continuous-flow analyzer (AutoAnalyzer, Type II) (II). In both, BAL-tributyrate is used as substrate, in combination with serum esterase inhibitors and a chromogenic reagent for the SH group of the liberated BAL. Serum lipase activities of patients with pancreatic diseases, measured at 90 or 40 samples per hour by I or II, respectively, correlated well with those measured by the corresponding manual method or by Kaplan's radioassay (Anal. Biochem. 33: 213, 1970). The correlation coefficients were all greater than 0.95, and the coefficients of variation were less than 8%, showing the practical usefulness of these procedures.
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