A simple liquid medium for the cultivation of Leishmania parasites is described. Leishmania brasiliensis and Leishmania peruviana cultured in this medium reached cell densities greater than 10(7) promastigotes per ml within 7 days. This medium compares very favorably with the more complex media used to cultivate Leishmania spp. and other hemoflagellates.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC272219 | PMC |
| http://dx.doi.org/10.1128/jcm.15.5.949-950.1982 | DOI Listing |
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