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DNA supercoiling enhances DNA condensation by ParB proteins.
Nucleic Acids Res
November 2024
Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, Van der Massweg 9, 2629HZ Delft, Netherlands.
The ParABS system plays a critical role in bacterial chromosome segregation. The key component of this system, ParB, loads and spreads along DNA to form a local protein-DNA condensate known as a partition complex. As bacterial chromosomes are heavily supercoiled due to the continuous action of RNA polymerases, topoisomerases and nucleoid-associated proteins, it is important to study the impact of DNA supercoiling on the ParB-DNA partition complex formation.
View Article and Find Full Text PDFMitochondrial Genome-Encoded Long Noncoding RNA (Lnc) and Mitochondrial Ribonucleases in Diabetic Retinopathy.
Biomedicines
July 2024
Ophthalmology, Visual and Anatomical Sciences, Wayne State University, 4717 St. Antoine, Detroit, MI 48201, USA.
Aim: Hyperglycemia damages mitochondria and downregulates transcription of mtDNA-encoded genes and the long noncoding RNA Lnc, causing mitochondrial genomic instability. The genes encoded by mtDNA are transcribed as large polycistronic transcripts, and the 5' ends of precursor tRNAs are processed by mitochondrial-targeted ribonuclease P (MRPPs). Our aim was to investigate the role of MRPP1 in the downregulation of Lnc in diabetic retinopathy.
View Article and Find Full Text PDFIn Vitro Transcription Assay to Quantify Effects of H-NS Filaments on RNA Chain Elongation by RNA Polymerase.
Methods Mol Biol
July 2024
Departments of Biochemistry, University of Wisconsin - Madison, Madison, WI, USA.
Bacterial chromosomal DNA is structured and compacted by proteins known as bacterial chromatin proteins (i.e., nucleoid-associated proteins or NAPs).
View Article and Find Full Text PDFTracking pairwise genomic loci by the ParB-ParS and Noc-NBS systems in living cells.
Nucleic Acids Res
May 2024
Gene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
The dynamics of genomic loci pairs and their interactions are essential for transcriptional regulation and genome organization. However, a robust method for tracking pairwise genomic loci in living cells is lacking. Here we developed a multicolor DNA labeling system, mParSpot (multicolor ParSpot), to track pairs of genomic loci and their interactions in living cells.
View Article and Find Full Text PDFThermus thermophilus is reportedly polyploid and carries four to five identical genome copies per cell, based on molecular biological experiments. To directly detect polyploidy in this bacterium, we performed live cell imaging by X-ray free-electron laser (XFEL) diffraction and observed its internal structures. The use of femtosecond XFEL pulses enables snapshots of live, undamaged cells.
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