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Optimization of polyhydroxyalkanoate (PHA) production from biohythane pilot plant effluent by Cupriavidus necator TISTR 1335.
Biodegradation
January 2025
Master's Program of Green Energy Science and Technology, Feng Chia University, Taichung City, 407102, Taiwan.
Bioplastics, particularly polyhydroxyalkanoates (PHAs), are emerging as promising alternatives to traditional materials due to their biodegradability. This study focuses on the production of PHAs as bioplastics using effluent from hydrogen production in a two-stage Biohythane Pilot Plant, which provides a low-cost substrate. The aim is to optimize production conditions, with Cupriavidus necator TISTR 1335 being used as the PHA producer.
View Article and Find Full Text PDFPredictors of pelvic acceleration during treadmill running across various stride frequency conditions.
Sports Biomech
January 2025
School of Sport and Health Sciences, Cardiff Metropolitan University, Cardiff, UK.
Pelvic running injuries often require extensive rehabilitation and pelvic girdle pain is a barrier to running engagement in population sub-groups, such as perinatal women. However, exploration into how external pelvic loading may be altered during running is limited. This study assessed which biomechanical variables influence changes in external peak pelvic acceleration during treadmill running, across various stride frequency conditions.
View Article and Find Full Text PDFEvaluation of Real-Time Cardiovascular Flow MRI Using Compressed Sensing in a Phantom and in Patients With Valvular Disease or Arrhythmia.
J Magn Reson Imaging
January 2025
Clinical Physiology, Department of Clinical Sciences Lund, Lund University, Skåne University Hospital, Lund, Sweden.
Background: Real-time (RT) phase contrast (PC) flow MRI can potentially be used to measure blood flow in arrhythmic patients. Undersampled RT PC has been combined with online compressed sensing (CS) reconstruction (CS RT) enabling clinical use. However, CS RT flow has not been validated in a clinical setting.
View Article and Find Full Text PDFEnabling icIEF Peak Identification of AAV Capsid Proteins by Fractionation on MauriceFlex and Subsequent Analysis by LC-MS.
Electrophoresis
January 2025
Pfizer, Analytical Research and Development, Chesterfield, Missouri, USA.
A significant limitation of imaged capillary electric focusing (icIEF) is the inability to identify and characterize specific species in the electropherogram. This has led to the development of complementary ion-exchange chromatography (IEX)-based methods that are amenable to either fraction collection and subsequent characterization or online IEX coupled to mass spectrometry. To overcome this limitation while maintaining the use of icIEF, novel approaches, including an icIEF separation and fractionation technology (MauriceFlex, ProteinSimple), have been developed.
View Article and Find Full Text PDFOnline integration of capillary electrophoresis and dual detector Taylor dispersion analysis a 3D printed instrument.
Analyst
January 2025
Department of Chemistry & Biochemistry, New Mexico State University, Las Cruces, NM, 88003-001, USA.
Hydrodynamic radius () is a descriptive metric of protein structure with the potential to impact drug development, disease diagnosis, and other important research areas of molecular biology. Common instrumental methods for molecular size characterization are disadvantageous due to high sample consumption, measurements made in non-physiological conditions, and/or inaccurate size determinations. Capillary Taylor dispersion analysis (TDA) is a molecular sizing method that utilizes nL sample volumes and achieves absolute size determination without calibration or comparison to standards.
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