Bacteria, yeasts and fungi suspended in a dextran solution were added to ampoules containing strips of filter paper which were dried without vacuum conditions. The ampoules were sealed and stored in the dark at room temperature. Viability counts were made of the original suspension immediately after drying and after storage periods of 3-48 months. Although bacterial cultures of many general did not show much resistance against dry conditions, bacteria of 13 other general had survived well or moderately after 4 years of storage. Most of the dried yeast cultures had survived after this period. Of the 16 fungal genera tested, species of 6 genera exhibited growth after 4 years. Results of this study were compared with those of two other preservation methods by which the same microorganisms were used.
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http://dx.doi.org/10.1007/BF00443240 | DOI Listing |
Braz J Biol
January 2025
Embrapa Agrobiologia, Seropédica, RJ, Brasil.
The objective was to evaluate the sensitivity of Piptadenia gonoacantha seeds to desiccation and storage conditions. The seeds were subjected to artificial drying in a forced air convection oven (39.7 °C ± 0.
View Article and Find Full Text PDFInt J Biomater
January 2025
Iranian Center for Endodontic Research, Research Institute for Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran 1983963113, Iran.
This study aimed to evaluate the impact of different manipulation methods and storage environments on the microstructural, chemical, and mechanical properties of calcium-enriched mixture (CEM) cement. Four sample groups were examined, including nondried (ND-I) and dried (D-I) groups placed directly in an incubator, dried samples stored in phosphate-buffered saline (PBS) (D-P), and dried samples stored in distilled water (D-W). Various analyses, including Vickers microhardness, compressive strength, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDS) were conducted after incubating the samples for 7 days.
View Article and Find Full Text PDFPeerJ
January 2025
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, Kazakhstan.
Background: Chitosan nanoparticles (CsNPs) are an effective and inexpensive approach for DNA delivery into live cells. However, most CsNP synthesis protocols are not optimized to allow long-term storage of CsNPs without loss of function. Here, we describe a protocol for CsNP synthesis, lyophilization, and sonication, to store CsNPs and maintain transfection efficiency.
View Article and Find Full Text PDFInt J Pharm
January 2025
Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, United States. Electronic address:
For monoclonal antibody drug products as for other biologics, while the innovator drug products first becomes commercially available, they are often followed by one or more biosimilar products. These biosimilars often differ from the innovator product, as well as from each other, in their formulation composition. However, the impact of the formulation composition on the stability of the active pharmaceutical ingredient subjected to different 'stresses' is still not understood.
View Article and Find Full Text PDFFoods
January 2025
Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy, National and Kapodistrian University of Athens, 15771 Athens, Greece.
The abundant yet underutilized olive leaves, a renewable by-product of olive cultivation, offer untapped potential for producing high-value bioactive compounds, notably oleacein. Existing extraction methods are often inefficient, yielding low quantities of oleacein due to enzymatic degradation of its precursor, oleuropein, during conventional processing and storage. This study aimed to overcome these limitations by exploring a novel methodology based on freeze-drying, to facilitate the in situ enzymatic biotransformation of oleuropein into oleacein directly within the plant matrix.
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