Macrophage migration inhibitory activity from Zajdela hepatoma ascites (AH-MIF) was characterized by ion exchange chromatography, gel filtration and isoelectric focusing. The data obtained indicate molecular heterogeneity according to net charge as revealed by chromatography on DEAE-Sephadex A-50. Furthermore, molecular sieve chromatography with Sephadex G-100 demonstrated the existence of subfractions with molecular weights of about 45,000, 20,000 and 10,000 - 15,000 respectively. Isoelectric focusing revealed further heterogeneity of a subfraction (10,000 - 15,000) eluting at 0.17 - 0.25 M NaCl by ion exchange chromatography. The migration inhibitory factor(s) from Zajdela hepatoma ascites were shown to adsorb to alpha-L-fucose-Sepharose 6B columns. The relationships of AH-MIF to functionally similar tumor-derived factors and to lymphocyte-derived conventional MIF ar discussed.
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