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Proximity-activated guide RNA of CRISPR-Cas12a for programmable diagnostic detection and gene regulation.
Nucleic Acids Res
January 2025
Beijing Key Laboratory for Bioengineering and Sensing Technology, University of Science and Technology Beijing, Beijing 100083, China.
The flexibility and programmability of CRISPR-Cas technology have made it one of the most popular tools for biomarker diagnostics and gene regulation. Especially, the CRISPR-Cas12 system has shown exceptional clinical diagnosis and gene editing capabilities. Here, we discovered that although the top loop of the 5' handle of guide RNA can undergo central splitting, deactivating CRISPR-Cas12a, the segments can dramatically restore CRISPR function through nucleic acid self-assembly or interactions with small molecules and aptamers.
View Article and Find Full Text PDFTheophylline use-a conundrum in modern medicine: a case report.
AME Case Rep
September 2024
Department of Nephrology, Appalachian Regional Healthcare, Harlan, KY, USA.
Background: The theophylline toxidrome presents with multisystemic involvement that includes cardiovascular, neurologic, metabolic, musculoskeletal, and gastrointestinal manifestations. Considering such a varied spectrum of presentations, it is often difficult to ascertain the diagnosis of this particular toxidrome. Review of home medications is an important step when working with a patient presenting as a toxidrome.
View Article and Find Full Text PDFLooking at the albumin-drug binding via partitioning in aqueous two-phase system.
Biochem Biophys Res Commun
January 2025
Cleveland Diagnostics, 3615 Superior Ave., Cleveland, OH, 44114, USA. Electronic address:
The partition coefficient of human serum albumin (HSA) was analyzed in the PEG600-Dex70, 0.15 M NaCl/KCl in 0.01 M Na/K phosphate buffer, pH 7.
View Article and Find Full Text PDFSystem biology analysis of miRNA-gene interaction network reveals novel drug targets in breast cancer.
Nucleosides Nucleotides Nucleic Acids
December 2024
School of Artificial Intelligence, Hangzhou Dianzi University, Hangzhou, China.
Article Synopsis
- Breast cancer is a complex disease that is very common globally, and while some treatments exist, there's a pressing need to find better drug targets.
- This study analyzed six sequencing datasets from the GEO database, identifying 67 miRNAs and 133 genes that are differentially expressed in breast cancer, and constructed a miRNA-gene interaction network.
- Among the identified molecules, three miRNAs were found to significantly impact patient prognosis, leading researchers to propose four potential drug candidates for targeted therapy in breast cancer treatment.
Blood metabolomic profiling reveals new targets in the management of psychological symptoms associated with severe alcohol use disorder.
Elife
November 2024
Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université catholique de Louvain, Brussels, Belgium.
Background: Alcohol use disorder (AUD) is a global health problem with limited therapeutic options. The biochemical mechanisms that lead to this disorder are not yet fully understood, and in this respect, metabolomics represents a promising approach to decipher metabolic events related to AUD. The plasma metabolome contains a plethora of bioactive molecules that reflects the functional changes in host metabolism but also the impact of the gut microbiome and nutritional habits.
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