The end of the logarithmic phase of growth of E. coli 113-3 cells in a mineral medium with methylcobalamin (MeCbl) occurs 60 minutes earlier than in the case of cells grown with cyancobalamin (CNCbl). Chromatography on a Sephadex - DEAE cellulose column of the 105 000xg fractions of cell-free extracts of the cells grown in the presence of MeCbl showed both quantitative and qualitative differences. The ability of individual protein fractions to transfer the 14CH3-groups from the Adomet to submethylated tRNA of E. coli K12W6 in the cells grown with MeCbl increases 1.5 - 3-fold in comparison with those grown with CNCbl. A chromatographic analysis of methylated bases of tRNA did not show any qualitative differences. In the presence of MeCbl the incorporation of the CH3-groups into m7G is increased by 50%.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis.
J Mater Sci Mater Med
January 2025
Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Implants aim to restore skeletal dysfunction associated with ageing and trauma, yet infection and ineffective immune responses can lead to failure. This project characterized the microbiological and host cell responses to titanium alloy with or without electroplated metallic copper. Bacterial viability counting and scanning electron microscopy quantified and visualized the direct and indirect bactericidal effects of the Cu-electroplated titanium (Cu-Ep-Ti) against two different Staphylococcus aureus strains.
View Article and Find Full Text PDFInterfacial Strain-Driven Large Topological Hall Effects in Supermalloy Thin Films with Noncoplanar Spin Textures.
ACS Appl Mater Interfaces
January 2025
School of Physical Sciences, Indian Association for the Cultivation of Science, 2A & 2B Raja S. C. Mullick Road, Kolkata 700032, India.
Materials exhibiting topological transport properties, such as a large topological Hall resistivity, are crucial for next-generation spintronic devices. Here, we report large topological Hall resistivities in epitaxial supermalloy (NiFeMo) thin films with [100] and [111] orientations grown on single-crystal MgO (100) and AlO (0001) substrates, respectively. While X-ray reciprocal maps confirmed the epitaxial growth of the films, X-ray stress analyses revealed large residual strains in the films, inducing tetragonal distortions of the cubic NiFeMo unit cells.
View Article and Find Full Text PDFSupercapacitor Cell Performance with Bacterial Nanocellulose and Bacterial Nanocellulose/Polybenzimidazole Impregnated Membranes as Separator.
Membranes (Basel)
January 2025
Institute of Electrochemistry and Energy Systems, Bulgarian Academy of Sciences, G. Bonchev Str. 10, 1113 Sofia, Bulgaria.
Supercapacitors are advanced energy storage devices renowned for their rapid energy delivery and long operational lifespan, making them indispensable across various industries. Their relevance has grown in recent years due to the adoption of environmentally friendly materials. One such material is bacterial nanocellulose (BNC), produced entirely from microbial sources, offering sustainability and a bioprocess-driven synthesis.
View Article and Find Full Text PDFExploring the Anticancer Potential of MonoHER (7-Mono-O-(β-Hydroxyethyl)-Rutoside): Mitochondrial-Dependent Apoptosis in HepG2 Cells.
Curr Issues Mol Biol
January 2025
The M-Lab, Department of Precision Medicine, GROW-Research Institute for Oncology and Reproduction, Maastricht University, 6200MD Maastricht, The Netherlands.
Background/aim: Flavonoids are a group of polyphenols, abundantly present in our diet. Although, based on their chemoprotective effects, intake of flavonoids is associated with a high anticancer potential as evidenced in in vitro and in vivo models, the molecular mechanism is still elusive. This study explores the antiproliferative and cytotoxic effects of the semi-synthetic flavonoid MonoHER (7-mono-O-(β-hydroxyethyl)-rutoside) in vitro on cancer cells.
View Article and Find Full Text PDFFunctional differentiation of human dental pulp stem cells into neuron-like cells exhibiting electrophysiological activity.
Stem Cell Res Ther
January 2025
Department of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Bizkaia, 48940, Spain.
Background And Aim: Human dental pulp stem cells (hDPSCs) constitute a promising alternative for central nervous system (CNS) cell therapy. Unlike other human stem cells, hDPSCs can be differentiated, without genetic modification, to neural cells that secrete neuroprotective factors. However, a better understanding of their real capacity to give rise to functional neurons and integrate into synaptic networks is still needed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!