The paper presents measurements of the difference in the melting temperature of a colE1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for .2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5x10(-5) for the cooperatively factor sigma. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC327533 | PMC |
| http://dx.doi.org/10.1093/nar/9.20.5469 | DOI Listing |
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