Methods are described for detection of Legionella pneumophila in cooling tower water or other water sources by direct fluorescent-antibody staining. A procedure for isolation of Legionella bacteria from water samples by guinea pig inoculation is described. Two different serogroups of L. pneumophila were isolated repeatedly from one of the cooling towers.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC243889PMC
http://dx.doi.org/10.1128/aem.41.5.1202-1205.1981DOI Listing

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