AI Article Synopsis
- Cultivating the yeast Pichia guilliermondii in high concentrations of sulfate or phosphate (0.6 M or more) makes it more vulnerable to actinomycin D and certain riboflavin analogs, while also reducing its need for external vitamin B2.
- Protoplasts of this yeast showed increased susceptibility to actinomycin D when exposed to high sulfate levels.
- Similar effects were observed in other yeast species and in E. coli under elevated phosphate conditions, suggesting that these ions can be used in experiments to examine the impact of transcription inhibitors like actinomycin D on specific enzyme inductions.
Article Abstract
Cultivation of the yeast Pichia guilliermondii in a medium with a high content of sulfate or phosphate ions (0.6 M and higher) increased its susceptibility to actinomycin D and 7-methyl-8-trifluoromethyl 10-(1'-D-ribityl)isoalloxazin, and analog of riboflavin, and decreased the requirement of the riboflavin-dependent mutant P7 in exogenous vitamin B2. The protoplasts of the yeast were also very susceptible to actinomycin D when they were incubated in a medium with a high sulfate concentration. Sulfate and phosphate ions elevated the susceptibility to actinomycin D in the following yeasts, apart from P. guilliermondii: Pichia pinus, Saccharomyces cerevisiae, Torulopsis candida, hansenula polymorpha, Schwanniomyces occidentalis, Candida utilis and Candida tropicalis. The growth of Escherichia coli was also very susceptible to actinomycin D when the bacterium was cultivated in medium with an elevated phosphate concentration (0.2 M). High phosphate or sulfate concentrations can be used in experiments aimed at studying the effect of transcription inhibitors (actinomycin D, 8-hydroxyquinoline) on the induction of alpha-glucosidase in P. guilliermondii.
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