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Peroxidase-antiperoxidase complex method (PAP) was used to reveal the CEA antigen in the larynx carcinoma cells by means of electron microscope. Studies were carried out on the material taken from 8 patients with the larynx carcinoma. In all cases positive immunoperoxidase reaction has been observed.
View Article and Find Full Text PDFIn the studied material taken from 70 patients with the mammary carcinoma was tried to discover the presence of the CEA antigen in the cells on the ultrastructural level. In studies was used peroxidase-antiperoxidase method (PAP). The product of the immunocytochemical reaction was found on the surface of cell membrane.
View Article and Find Full Text PDFThe effect of melanin pigment removal on the peroxidase-antiperoxidase immunoperoxidase technic.
Am J Clin Pathol
October 1987
Department of Histopathology, East Birmingham Hospital, Bordesley Green East, England.
With the increasing use of immunoperoxidase technics, it may be difficult to differentiate between the dark staining of 3,3'-diaminobenzidine (DAB) compound reaction product and melanin pigment. The latter may be particularly observed in skin. Samples of both normal skin and melanotic malignant melanoma were treated for the removal of melanin by standard technics both before and after a peroxidase-antiperoxidase (PAP) immunohistologic sequence.
View Article and Find Full Text PDFThe avidin-biotin-peroxidase-antiperoxidase complex method (ABPAP) of immunohistochemistry employs the sequential application of the peroxidase-antiperoxidase complex (PAP) and the avidin-biotin-peroxidase complex (ABC) technics. To assess the efficacy of ABPAP in the detection of cellular antigens and to compare its utility with that of protease digestion technics, the authors studied cytokeratin (CK) reactivity in six examples each of colon, prostate, and breast cancer and Leu-M1 reactivity in five ductal breast carcinomas. ABC, PAP and ABPAP were applied to these cases alone and in combination with prior pepsin digestion of deparaffinized sections.
View Article and Find Full Text PDFPlasma cell cytoplasmic immunoglobulin was stained using the peroxidase-antiperoxidase technic in Bouin-fixed, paraffin-embedded human tissues from different origins. Bone marrow (BM), tonsils, and appendices were examined. IgA-, IgD-, and IgM-secreting plasmocytes were easily studied using highly diluted rabbit antihuman antisera in all tissues, including BM.
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