Preparation of zymosan from yeast cell walls.

Folia Microbiol (Praha)

Published: February 1981

Cell walls of the yeast Saccharomyces cerevisiae after disintegration and protoplasm removal by centrifugation and repeated washing were suspended in 0.5 M Na2HPO4, pH 7.8-8.0, as a 5% or 10% suspension, depending on the mode of heating. The suspension was boiled for 3 h, purified by repeated washing with water and ethanol and dried. The yield was approximately 1.8% of the starting amount of pressed commercial baker's yeast.

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http://dx.doi.org/10.1007/BF02897218DOI Listing

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