Legionnaires' disease serology. Effect of antigen preparation on specificity and sensitivity of the indirect fluorescent antibody test.

Sera from 31 Legionnaires' disease (LD) survivors of the Philadelphia outbreak, 31 Legionnaire (L) controls, and 300 additional controls were examined for the presence of specific antibodies to five antigen preparations of Legionella pneumophila (serogroup 1) to determine the effect of antigen preparation on the sensitivity and specificity of the indirect immunofluorescence test. Diagnostic levels were determined for each antigen at the upper limit of normal value (ULNV) titre, which established the titre not exceeded by 85% of controls. Antigens were prepared from formalin-killed L. pneumophila suspended in egg yolk sac (EYS) (LPF:EYS) or bovine serum albumin (BSA) (LPF:BSA); and from heat-killed organisms suspended in EYS (LPH:EYS) or BSA (LPH:BSA). Antigen was also supplied by the Center for Disease Control (CDC:AG).Although there was wide variation in the sensitivity of the antigens, at the ULNV level all antigens tested could be used to differentiate LD survivors from L controls (p<0.001; X(2) test). Formalin treatment resulted in the most specific antigen by eliminating titres in L controls. The results of the X(2) test, comparing LD survivors with L controls, ranked the antigens in the following ascending order of sensitivity: LPH:BSA 15.3,