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Omega (ω)-3 fatty acids (FAs) are essential components of cell membranes that also serve as precursors of numerous regulatory molecules. α-linolenic acid (ALA), one of the most important ω3 FAs in plants, is synthesized in both the plastid and extraplastidial compartments. FA Desaturase (FAD) 3 is an extraplastidial enzyme that converts linoleic acid (LA) to ALA.

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Nitrous oxide production via enzymatic nitroxyl from the nitrifying archaeon .

Proc Natl Acad Sci U S A

January 2025

Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, NY 14853.

Ammonia oxidizing archaea (AOA) are among the most abundant microorganisms on earth and are known to be a major source of nitrous oxide (NO) emissions, although biochemical origins of this NO remain unknown. Enzymological details of AOA nitrogen metabolism are broadly unavailable. We report the recombinant expression, purification, and characterization of a multicopper oxidase, Nmar_1354, from the AOA .

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Background: Geraniol 10-hydroxylase (G10H) is a cytochrome P450 monooxygenase involved in regulation, which is involved in the biosynthesis of monoterpene. However, G10H is not characterized at the enzymatic mechanism and regulatory function in .

Methods And Results: A gene related to the biosynthesis of monoterpenoid, geraniol 10-hydroxylase, has been cloned from the medicinal plant .

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Cellulose-derived biomaterials offer a sustainable and versatile platform for various applications. Enzymatic engineering of these fibers, particularly using lytic polysaccharide monooxygenases (LPMOs), shows promise due to the ability to introduce functional groups onto cellulose surfaces, potentially enabling further functionalization. However, harnessing LPMOs for fiber engineering remains challenging, partly because controlling the enzymatic reaction is difficult and partly because limited information is available about how LPMOs modify the fibers.

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Herein, novel thiazolo[4,5-]quinoxalin-2-ones 2-6 and thiazolo[4,5-]quinoxalin-2(3)-imines 7-9 were synthesized and characterized using elemental analysis, IR spectroscopy, and H/C NMR to confirm their structures. The efficacy of the newly designed thiazolo-quinoxalines 2, 3, 4, 5, 7, 8, and 9 against the cotton leafworm (2nd and 4th instar larvae) was evaluated, and results revealed insecticidal activity with variable and good mortality percentages. A SAR study was also discussed.

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