Prothymocytes were present in long-term marrow cell cultures for 17 weeks, although at very low levels. The prothymocytes from the cultures appeared normal in their thymus repopulation ability since the growth curves for thymocytes derived from cultured cells, measured in thymuses of irradiated mice, were parallel to those for thymocytes derived from fresh bone marrow prothymocytes. The buoyant density distribution for prothymocytes from the cultures appeared normal as well, with a modal density of 1,069 g/cm3. The prothymocyte density profile was similar to that for CFUS from the cultures which had a modal density of 1.070 g/cm3. Prothymocytes were lost from culture before CFUS and a decreased ratio of prothymocytes to CFUS was evident within the first 24 hours of culture. The kinetic differences between prothymocytes and CFUS in vitro suggest that these two functional assays detect different cells.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Intrathymic transplantation of bone marrow-derived progenitors provides long-term thymopoiesis.
Blood
March 2010
Institut de Génétique Moléculaire de Montpellier, Centre National de la Recherche Scientifique (CNRS) UMR 5535/IFR 122, Montpellier, France.
The sustained differentiation of T cells in the thymus cannot be maintained by resident intrathymic (IT) precursors and requires that progenitors be replenished from the bone marrow (BM). In patients with severe combined immunodeficiency (SCID) treated by hematopoietic stem cell transplantation, late T-cell differentiation defects are thought to be due to an insufficient entry of donor BM progenitors into the thymus. Indeed, we find that the intravenous injection of BM progenitors into nonconditioned zeta-chain-associated protein kinase 70 (ZAP-70)-deficient mice with SCID supports short- but not long-term thymopoiesis.
View Article and Find Full Text PDFIn vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer.
J Clin Invest
August 2005
Institut de Génétique Moléculaire de Montpellier, CNRS UMR 5535/Institut Fédératif de Recherch 122, Montpellier, France.
SCID patients have been successfully treated by administration of ex vivo gene-corrected stem cells. However, despite its proven efficacy, such treatment carries specific risks and difficulties. We hypothesized that some of these drawbacks may be overcome by in situ gene correction of T lymphoid progenitors in the thymus.
View Article and Find Full Text PDFCharacterization of thymic progenitors in adult mouse bone marrow.
J Immunol
February 2003
Department of Pathology, University of Utah, 30 North 1900 East, Salt Lake City, UT 84132, USA.
Thymic cellularity is maintained throughout life by progenitor cells originating in the bone marrow. In this study, we describe adult mouse bone cells that exhibit several features characteristic of prothymocytes. These include 1) rapid thymic engraftment kinetics following i.
View Article and Find Full Text PDFPrethymic expression of a transgenic TCR beta chain on a precursor of T-cells.
Cell Immunol
October 1997
Department of Pathology, University of Florida College of Medicine, Health Sciences Center, Gainesville, Florida, 32610, USA.
Mice carrying a rearranged TCR Vbeta 8.2 transgene express the Vbeta protein on the vast majority of peripheral T-cells. The bone marrow and peripheral blood, as well as other lymphoid organs of both untreated animals and animals depleted of T-cells by neonatal thymectomy and/or injection from birth of monoclonal anti-TCR antibodies, contain a small population of cells that express low levels of the Vbeta transgene product, but no T-cell or other detectable lineage-specific phenotypic markers.
View Article and Find Full Text PDFPurified murine long-term in vivo hematopoietic repopulating cells are not prothymocytes.
Exp Hematol
January 1995
Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Victoria, Australia.
Analysis of kinetics of thymic repopulation by Rh123low, Lin-, Ly6A/E+, c-kit+ (Rh123low) cells, highly enriched for long-term in vivo hematopoietic repopulating cells, reveals that this population is deficient in thymic repopulation at week 3 after intravenous transplantation when compared to normal bone marrow cells. This suggests that the marrow prothymocytes have been depleted from this population, and analysis of thymic repopulation at week 3 can therefore be used to differentiate prothymocytes and their precursors. Using this short-term assay, the Rh123high, Lin-, Ly6A/E+, c-kit+ (Rh123high) population has been found to be relatively more efficient at early thymic repopulation, suggesting that this population contains the prothymocytes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!