AI Article Synopsis
- A new micromethod for measuring 17 alpha-hydroxyprogesterone in blood on filter paper has been created, which is fast, simple, and just as accurate as traditional methods.
- This method has been specifically tested for screening congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency, showing good results compared to another established tracer method.
- The method successfully diagnosed CAH in a neonate, indicating its potential as an effective screening tool, though more infant studies are needed to fully assess its advantages and disadvantages.
Article Abstract
A micromethod for measuring 17 alpha-hydroxyprogesterone in blood collected on filter paper has been developed. Our method is rapid, easy and has the specificity, accuracy and precision of the radioimmunoassay in whole blood. The method has been applied for screening patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. Fifty samples collected on filter paper were assayed by our method, using 125I as tracer, and results were compared with those obtained for the same samples using a tritium tracer. The agreement between the two methods was particularly good in the area ranging from 15 to 100 pg/disc. In one neonate the diagnosis of CAH was made utilizing the microfilter paper method. Our method is a promising screening test for CAH. An indication of the advantages or disadvantages of this type of screening will become available when an adequate number of infants has been examined.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF03350496 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!