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Systematic comparison of quantity and quality of RNA recovered with commercial FFPE tissue extraction kits.
J Transl Med
January 2025
Tumour Biology and Immunology Laboratory, Research Branch, Sidra Medicine, Doha, Qatar.
Background: FFPE tissue samples are commonly used in biomedical research and are a valuable source for next-generation sequencing in oncology, however, extracting RNA from these samples can be difficult the quantity and quality achieved can impact the downstream analysis. This study compared the effectiveness of seven different commercially available RNA extraction kits specifically designed for use with FFPE samples in terms of the quantity and quality of RNA recovered.
Methods: This study used 9 samples of FFPE tissue from three different types of tissue (Tonsil, Appendix and lymph node of B-cell lymphoma) to evaluate RNA extraction methods.
An Observational Study on the Analysis of Vitamin D Deficiency in COVID-19 Patients With No Comorbidities: A Retrospective Analysis.
Cureus
November 2024
Internal Medicine, Max Smart Super Specialty Hospital, New Delhi, IND.
Introduction: Vitamin D deficiency is an important problem when facing a viral disease. Vitamin D deficiency is widely prevalent in India and plays an important role in immunoregulation. The deficiency can lead to severe viral infections.
View Article and Find Full Text PDFThe immune cells profiles of individuals with sulfur mustard-induced serious long-term respiratory complications.
Int Immunopharmacol
December 2024
Department of Immunology, Tarbiat Modares University, Tehran, Iran.
Sulfur mustard (SM) induced pulmonary disorder is a heterogeneous disease characterized by uncontrolled inflammatory immune responses. In this cross-sectional study carried out in Isfahan-Iran, our objective was to thoroughly evaluate the clinical health and peripheral blood leukocyte profiles of adult veterans exposed to SM 25-30 years. In total, 361 people were studied in two groups, 287 chemical veterans with pulmonary complications and 64 healthy individuals as a control group.
View Article and Find Full Text PDFImmune modulation of buffalo peripheral blood mononuclear cells by two asparaginyl endopeptidases from Fasciola gigantica.
Parasit Vectors
December 2024
College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi Zhuang Autonomous Region, People's Republic of China.
Background: Fascioliasis is a zoonotic parasitic disease caused by Fasciola hepatica and Fasciola gigantica, which poses a serious threat to global public health and livestock farming. Fasciola gigantica secretes and excretes various components to manipulate the immune response, thereby enhancing its invasion, migration, and survival in vivo. However, the roles of specific components in immune modulation, such as asparagine endopeptidase, remain unknown.
View Article and Find Full Text PDFAssociation of hormone profile with various respiratory disorders in sulfur mustard-chemical veterans.
Int Immunopharmacol
December 2024
Immunoregulation Research Center, Shahed University, Tehran, Iran. Electronic address:
Backgrounds: Chronic respiratory complications such as asthma, bronchiolitis obliterans, and chronic bronchitis are prevalent among individuals exposed to sulfur mustard (SM). This study investigates the hormonal changes associated with SM-induced respiratory disorders.
Methods: A cross-sectional analysis was conducted involving 276 male participants exposed to SM during the Iraq-Iran war and 64 healthy controls.
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