Effectiveness of some anions in sustaining the efferent inhibition in the frog labyrinth.

Efferent inhibition in the frog labyrinth is sustained by the release of acetylcholine (ACh) which opens a Cl(-)-channel in the hair cell membrane. To investigate more closely the nature of the permeability change underlying the ACh reaction, the external Cl(-) was replaced by anions of increasing hydrated size, and to test the possible role of a Cl(-)-pump in the sensory cells, drugs were applied which are known to block active cl(-) pumping in other systems. Experiments indicate that the ACh-operated inhibitory channel of the hair cell is larger than at other inhibitory synapses (or approximately 0.7 nm), while pharmacological treatments (DNP, NaN3, acetazolamide, ammonium acetate, DIDS) fail to demonstrate any active distribution of Cl(-) across the hair cell membrane.