AI Article Synopsis
- The human promyelocytic leukaemia cell line HL60 was analyzed to understand its growth patterns, revealing an exponential proliferation phase up to a saturation density of approximately 3.0 x 10(6) cells/ml.
- The study calculated a doubling time of 34.0 hours and detailed cell cycle parameters, showing an average total duration of 24.3 hours, with specific phases lasting G1: 3.8 hr, S: 15.1 hr, and G2: 4 hr.
- The growth fraction was found to be 0.85, indicating that most of the cells were actively dividing, while cell loss primarily occurred in quiescent cells, positioning HL60 as a valuable model for studying
Article Abstract
In order to characterize the growth pattern of the human promyelocytic leukaemia cell line HL60, its kinetic parameters were studied. The doubling time was calculated from serial cell counts, the duration of the various cell cycle phases from the analysis of the labelled mitoses curve, and quiescent population from continuous labelling experiments. Proliferation in culture was exponential up to a saturation density of about 3.0 x 10(6) cells/ml, with a doubling time of 34.0 hr. The cell cycle duration was 24.3 +/- 4.1 hr (SD), and that of the cell cycle phases was: G1, 3.8 +/- 2.2 hr; S, 15.1 +/- 3 hr; and G2, 4 +/0 1.2 hr. The growth fraction was 0.85, and cell loss was restricted to the quiescent cells. The HL60 cell line, with fully characterized kinetics, provides a useful tool for the vitro study of substances which may affect human leukaemic myelopoietic proliferation.
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| http://dx.doi.org/10.1111/j.1365-2184.1982.tb01057.x | DOI Listing |
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