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Corneal nerve abnormalities in early-stage diabetic retinopathy evaluated by corneal confocal microscopy.
BMC Ophthalmol
January 2025
Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221006, China.
Objective: This study aims to investigate the correlation between the development of diabetic retinopathy (DR) and the changes in corneal sub-basal nerve plexus (SNP) and corneal dendritic cells (DCs).
Methods: 58 patients with type 2 diabetes mellitus (T2DM) and 30 age- and sex-matched healthy participants underwent assessment of the corneal nerve. The DR group was divided into no diabetic retinopathy (NDR) and 29 eyes with mild to moderate non-proliferative diabetic retinopathy (NPDR).
Claudin-11 regulates immunological barrier formation and spermatogonial proliferation through stem cell factor.
Commun Biol
January 2025
Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Tight junctions (TJs) between adjacent Sertoli cells are believed to form immunological barriers that protect spermatogenic cells expressing autoantigens from autoimmune responses. However, there is no direct evidence that Sertoli cell TJs (SCTJs) do indeed form immunological barriers. Here, we analyzed male mice lacking claudin-11 (Cldn11), which encodes a SCTJ component, and found autoantibodies against antigens of spermatocytes/spermatids in their sera.
View Article and Find Full Text PDFBackground: Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal cancer with a 5-year survival rate of 12%. It has two major molecular subtypes: classical and basal, regulated by the master transcription factors (MTFs) GATA6 and ΔNp63, respectively.
Objective: This study sought to uncover the transcriptional regulatory mechanisms controlling PDAC subtype identity.
Human enteroid monolayers: A novel, functionally stable model for investigating oral drug disposition.
Drug Metab Dispos
January 2025
Department of Pharmaceutics, School of Pharmacy, University of Washington, Seattle, Washington. Electronic address:
To further the development of an in vitro model that faithfully recapitulates drug disposition of orally administered drugs, we investigated the utility of human enteroid monolayers to simultaneously assess intestinal drug absorption and first-pass metabolism processes. We cultured human enteroid monolayers from 3 donors, derived via biopsies containing duodenal stem cells that were propagated and then differentiated atop permeable Transwell inserts, and confirmed transformation into a largely enterocyte population via RNA sequencing analysis and immunocytochemistry (ICC) assays. Proper cell morphology was assessed and confirmed via bright field microscopy and ICC imaging of tight junction proteins and other apically and basolaterally localized proteins.
View Article and Find Full Text PDFThe importance of thiamine availability in the thermogenic competency of human adipocytes.
Mol Cell Endocrinol
January 2025
Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, H-4032, Debrecen, Hungary. Electronic address:
Brown and beige adipocytes express uncoupling protein-1 (UCP1), which is located in the inner mitochondrial membrane and facilitates the dissipation of excess energy as heat. The activation of thermogenic adipocytes is a potential therapeutic target for treating type 2 diabetes mellitus, obesity, and related co-morbidities. Therefore, identifying novel approaches to stimulate the function of these adipocytes is crucial for advancing therapeutic strategies.
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