The transport of radiolabelled rat submandibular gland kallikrein was studied after local administration to the resting and activated rat submandibular gland. The iodinated kallikrein was electrophoretically, immunologically, and biologically indistinguishable from the intact enzyme. After intraductal and intraglandular application the radioactivity in venous effluent was quantitated and characterized. As judged by gel-filtration 125I-kallikrein in venous effluent eluted at a position similar to that seen when the iodinated enzyme was mixed with plasma, but earlier than the elution of 125I-kallikrein in buffer. In plasma, therefore, glandular kallikrein is probably bound to macromolecules. The radioactive fractions in venous effluent did not contain free iodine. Maximum concentration of 125I-kallikrein in venous effluent of resting glands was repeatedly reached about 20 min after intraductal administration. Moreover, the ductal epithelium represented the main permeation barrier since after intraglandular application the maximum venous 125I-kallikrein concentration was reached almost immediately. In activated gland (parasympathetic and sympathetic nerve stimulation), the venous 125I-kallikrein concentration was inversely related to glandular blood flow. We conclude that kallikrein present in the duct lumen or in the interstitium is able to reach the circulation, thereby making possible the local generation of plasma-kinins.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1748-1716.1980.tb06602.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effect of Antithrombin Leakage From Hemodialysis Therapy on Antithrombin Hemodynamics.
Cureus
December 2024
Clinical Laboratory Science, Graduate School of Medical Science, Kanazawa University, Kanazawa, JPN.
Introduction Hemodialysis (HD) therapy is a crucial treatment for patients with renal failure but can impact the hemodynamics of antithrombin (AT), a protein essential for regulating hemostasis and preventing thrombosis. Reduced AT activity can lead to thrombus formation at unusual sites and increase the risk of recurrent venous thromboembolism. The loss of AT during HD or hemodiafiltration (HDF) through leakage or adsorption onto dialysis membranes has not been fully investigated, and its effects on AT hemodynamics remain unclear.
View Article and Find Full Text PDFImpact of pediatric continuous renal replacement therapy parameters on meropenem, piperacillin, and tazobactam pharmacokinetics: an model.
Expert Opin Drug Metab Toxicol
December 2024
UMR 1343, Evaluation des thérapeutiques et pharmacologie périnatale et pédiatrique, INSERM, Université Paris Cité, Paris, France.
Background: Limited data exist on how continuous renal replacement therapy (CRRT) affects antimicrobial dosing in pediatric patients. This study examined the impact of pediatric CRRT parameters on the pharmacokinetics (PK) of meropenem, piperacillin, and tazobactam using an in vitro CRRT model.
Research Design And Methods: An in vitro CRRT model with a pediatric ST60 circuit was used to assess antimicrobial clearance during continuous veno-venous hemodialysis (CVVHD) or hemofiltration (CVVH).
Edoxaban pharmacokinetics during in vitro continuous renal replacement therapy.
BMC Nephrol
October 2024
Department of Pharmacy Practice, College of Pharmacy, MHPE, BCPS - AQ Cardiology, FCCM, FCCP, University of Illinois at Chicago, 833 South Wood Street, Room 164 (M/C 886), Chicago, IL, 60612, USA.
Article Synopsis
- - Edoxaban was tested for its clearance during continuous renal replacement therapy (CRRT) by analyzing its levels in pre- and post-filter blood samples, using methods like liquid chromatography for accurate measurement.
- - The study found that edoxaban clearance was primarily due to its adsorption to specific hemofilters, with a confirmed average protein binding of 41%, and multivariate analysis showed that the CRRT setup had little effect on clearance rates.
- - The recommended dosing for edoxaban is 30-45 mg once daily, depending on the flow rate of the CRRT system, to achieve the necessary drug exposure for treating venous thromboembolism.
A shrapnel migration from a peripheral vein to the right ventricle: case report.
Eur Heart J Case Rep
September 2024
Department of Diagnostic Imaging, Rabin Medical Center, Beilinson Hospital, Tel Aviv University, Petah Tikva 49100, Israel.
Background: Foreign bodies that migrate into the heart may include medical devices dislodged from their original location or, rarely, external particles (shrapnel and other foreign bodies) that penetrate the vein, remain intraluminal, and migrate via the venous blood flow to the right heart. Most reported entry sites of these external foreign bodies were in the torso, thigh, or neck; none of them penetrated through a distal extremity of the body. We report a case where shrapnel was found in the right ventricle (RV) following penetrating injury to the hand.
View Article and Find Full Text PDFBiochemical, pathohistological, radiographic and cardiological analysis reveals the possible association between apical periodontitis and cardiac function in diabetic rats.
Arch Oral Biol
January 2025
Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic Disorders, 69 Svetozara Markovica St., 34000 Kragujevac, Serbia; Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, 69 Svetozara Markovica St., 34000 Kragujevac, Serbia; Department of Human Pathology, I.M. Sechenov First Moscow State Medical University, 8 Trubetskaya St., 119991 Moscow, Russian Federation.
Objective: To evaluate the possible effects of apical periodontitis (AP) on cardiac function, structure, and oxidative stress (OS) in rats with diabetes mellitus type 2 (T2DM).
Design: Forty-eight (Wistar albino, male) rats were randomized into four groups: control healthy (CTRL), normoglycemic with AP (AP), T2DM, and T2DM with AP (T2DM+AP). T2DM was induced by streptozotocin and a high-fat diet.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!