The sequence of 3'-termini of mRNAs from early region III of adenovirus 2.

The cloned cDNA sequences corresponding to early region III transcripts (76.6-86.0 map units) of adenovirus 2 were analyzed with a view to determine the structure of their 3'-termini. The clones pE7, pE22 and pE34, which contain a part of or the entire coding sequence for the Mr 19000 glycoprotein, showed microheterogeneity with respect to the poly(A) addition site, with the polyadenylation occurring 17, 26, or 29 nucleotides downstream from the hexanucleotide, 5'-ATTAAA-3'. The clone pE17 which represents a copy of the mRNA ending at 86.0 map units showed the presence of the sequence 5'-AATAAA-3', 16 nucleotides before the poly(A) tail.