1. The uptake, binding and elimination of phalloidin in liver is compared in adult (180 to 240 g) and "baby" (17 to 19 days old) rats in vivo and in vitro. 2. In both groups there is no relation between the concentration of the poison in the liver and the toxicity. 3. Although baby rats show a significantly higher tolerance against phalloidin than the adult animals, the concentration of the poison in the liver of baby rats is higher, and the elimination is significantly slower than in adult rats. 4. The very tight binding and concentration of phalloidin in the liver is explained by an extremely low dissociation constant. 5. Furthermore, the morphological differences between the poisoning of the liver cells in the entire organ and of isolated liver cells are discussed.
Download full-text PDF |
Source |
---|
Int J Mol Sci
December 2024
Goethe University, Frankfurt University Hospital, Medical Clinic 1, 60596 Frankfurt, Germany.
This study demonstrates the effectiveness of propidium iodide as a reliable marker for detecting dead or dying cells in frozen liver tissue sections. By comparing propidium iodide staining with the widely used Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, both methods showed consistent results in disease models such as alcohol-induced fibrosis and Western diet-induced fatty liver. Additionally, propidium iodide was successfully co-stained with other fluorescent markers, like phalloidin (for actin filaments) and antibodies targeting collagen, enabling detailed spatial analysis of dying cells within tissue.
View Article and Find Full Text PDFSLAS Discov
January 2025
Department of Pharmaceutical Sciences, School of Pharmacy, University of Pittsburgh, Pittsburgh, PA 15261, USA; University of Pittsburgh Medical Center Hillman Cancer Center, Pittsburgh, PA 15232, USA. Electronic address:
Biochimie
February 2025
Instituto de Ciencias Biológicas y Biomédicas del Sur (INBIOSUR), Universidad Nacional del Sur (UNS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Bahía Blanca, Argentina; Departamento de Biología, Bioquímica y Farmacia, UNS, San Juan 670, 8000, Bahía Blanca, Argentina. Electronic address:
J Appl Toxicol
February 2024
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, India.
Hepatocellular carcinoma (HCC) contributes to more than 80% of all primary cancers globally and ranks fourth in cancer-related deaths, due to the lack of an effective, definite therapeutic drug. Coleus vettiveroides (CV) has been used in Indian traditional medicine to treat diabetes, liver ailments, skin diseases, leukoderma, and leprosy. This study investigates the anticancer effect of CV ethanolic root extract in HepG2 cells.
View Article and Find Full Text PDFFood Chem Toxicol
September 2023
College of Pharmacy and BK21 Four-sponsored Advanced Program for SmartPharma Leaders, The Catholic University of Korea, Bucheon, 14662, Republic of Korea. Electronic address:
Phalloidin, a bicyclic heptapeptide found in Amanita mushroom, specifically binds to F-actin in the liver causing cholestatic hepatotoxicity. However, the toxicokinetics and tissue distribution properties of phalloidin as well as their underlying mechanisms have to be studied further. The area under the plasma concentration curve (AUC) of phalloidin increased in proportion to the doses (0.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!